中药血清对体外培养脐静脉内皮细胞增殖的影响

目的观察中药对内皮细胞增殖的抑制作用.方法制备含中药的大鼠血清,将其加入到培养的内皮细胞中,应用流式细胞仪观察细胞增殖与凋亡的情况.结果含中药血清对内皮细胞周期有明显影响,可使内皮细胞周期中G2-M期细胞比例减少,S期细胞相对增加,说明中药可阻滞内皮细胞由S期进入G2-M期,从而降低其有丝分裂能力,并可诱导内皮细胞凋亡.结论中药对内皮细胞的增殖有抑制作用,为临床治疗视网膜血管增生性病变提供实验依据.

Effect of serum containing traditional Chinese herb on proliferation of the cultured vascular endothelial cells

OBJECTIVE In order to observe the effect of traditional Chinese herb on anti-proliferation of vascular endothelia cell METHODS The serum of containing traditional Chinese herb was prepared by take a blood sample from rats The serum was gone into the cultured endothelial cells Then we investigate the effect on proliferation of vascular endothelial cells and apoptosis by the method of methyl thiazolyl tetrazolium(MTT)and flow cytometry (FCM) RESULTS The serum containing traditional Chinese herb has an effective inhibition on proliferation of the cultured vascular endothelial cells The serum hindered the transformation of vascular endothelial cells from S phase to G2-M phase The serum could induce apoptosis in cultured vascular endothelial cells CONCLUSION traditional Chinese herb may play an inhibiting role in proliferation of the cultured vascular endothelial cells This result could provide some experimental evidences for clinical treatment of proliferative vascular retinopathy