hSav1蛋白高表达对Mst1介导的细胞凋亡的影响 |
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引用本文: | 李兆明,刘韦成,董硕,罗学来,李小兰,陶德定,龚建平,胡俊波.hSav1蛋白高表达对Mst1介导的细胞凋亡的影响[J].中华肿瘤杂志,2005,31(1):481-484. |
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作者姓名: | 李兆明 刘韦成 董硕 罗学来 李小兰 陶德定 龚建平 胡俊波 |
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作者单位: | 华中科技大学同济医学院附属同济医院肿瘤研究所,武汉,430030; |
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基金项目: | 教育部新世纪优秀人才支持计划国家自然科学基金 |
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摘 要: | Objective To elucidate the effect of hSavl expression on Mstl-mediated apoptosis in HeLa cells. Methods Plasmids pCMV-HA-hSav1 and pcDNA/4TO-Flag-Mst1 were constructed and cotransfected into HeLa cells. Triple immunofluorescent labeling of hSav1, Mst1 and nucleus was performed to determine their subcellular localization. Plasmids pCMV-HA-hSav1 and/or pcDNA/4TO-Flag-Mst1 were transfected into HeLa cells, and 36 hours later cisplatin (50 μmol/L) as a pro-apoptotic agent was added for 14 hours. Cell apoptosis was analyzed by annexin V/PI assay. Results Plasmids pCMV-HA-hSav1 and pcDNA/4TO-Flag-Mst1 were constructed and the authenticity of constructs was verified by sequencing. The binding in vitro showed that hSavl could be detect from the anti-Mstl immunoprecipitation complex. The immunofluorescent labeling showed that hSavl and Mstl had the same localization in cells. Overexpressed protein hSavl did not induce a significant cell apoptosis. However, co-expression of hSavl with Mstl resulted in a significant increase of apoptosis above the level seen with Mstl alone (24. 5% ± 2.4% vs. 39.3% ± 4.0%, P < 0. 05). Conclusion Our findings indicate that hSavl is a newly identified protein that interacts with Mst1 and a, augments Mst1-mediated apoptosis.
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关 键 词: | 蛋白相互作用 细胞凋亡 HeLa细胞 |
Overexpression of hSav1 promotes Mst1-indneed apoptosis in HeLa cells |
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Abstract: | |
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Keywords: | hSav1Mst1Human salvador 1Mammalian sterile 20-like kinase 1Protein interactionsApoptosisHeLa cells |
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