外消旋雌马酚及其对映异构体对大肠癌HCT-15细胞增殖的影响

目的探讨外消旋雌马酚及雌马酚对映异构体对大肠癌HCT-15细胞增殖的影响及其机制。方法利用MTT法检测不同剂量(0、0. 5、1、5和10μmol/L)外消旋雌马酚和雌马酚对映异构体对大肠癌HCT-15细胞增殖的影响,蛋白免疫印迹法检测雌激素受体(estrogen receptor,ER)和核因子红系2相关因子2[nuclear factor(erythroid-derived 2)-like 2,Nrf2]的表达。结果外消旋雌马酚和(R)雌马酚可抑制HCT-15细胞增殖,且呈剂量-效应关系,而(S)雌马酚对HCT-15细胞增殖没有影响。外消旋雌马酚可增加ERβ和Nrf2表达,(R)雌马酚可增加Nrf2表达。结论外消旋雌马酚可通过雌激素效应和抗氧化效应抑制HCT-15细胞增殖,(R)雌马酚可通过抗氧化效应抑制HCT-15细胞增殖,而(S)雌马酚对HCT-15细胞增殖没有影响。

Effects of equol on proliferation of colorectal cancer HCT-15 cell

OBJECTIVE To investigate the effect of racemic equol and equol enantiomers on the proliferation of colorectal cancer HCT-15 cell and the potential mechanism. METHODS 3-( 4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide( MTT) assay was used to detect the effect of different concentrations of racemic equol and equol enantiomers( 0,0. 5,1,5 and 10 μmol/L) on the proliferation of colorectal cancer HCT-15 cell. Western blot was used to detect the expression of estrogen receptor( ER)and nuclear factor 2 related factor 2( Nrf2). RESULTS Racemic equol and( R) equol inhibited the proliferation of HCT-15 cell in a dose-dependent manner,whereas( S) equol had no effect on the proliferation of HCT-15 cell. Racemic equol increased the expression of ERβ and Nrf2,while( R) equol increased the expression of Nrf2. CONCLUSION Racemic equol can inhibit the proliferation of HCT-15 cell through estrogenic and antioxidative activity. R equol can inhibit the proliferation of HCT-15 cell through antioxidative activity,while( S) equol has no effect on the proliferation of HCT-15 cell.