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| 红鲫C1HD近交系RAPD标记的建立 | |
| 引用本文： | 田智,吴端生,郑济芳,江千秋.红鲫C1HD近交系RAPD标记的建立[J].中国比较医学杂志,2007,17(10):589-591. |
| 作者姓名： | 田智 吴端生 郑济芳 江千秋 |
| 作者单位： | 1. 南华大学生命科学与技术学院;长沙医学院,长沙,410219 2. 南华大学生命科学与技术学院;南华大学实验动物学部,湖南衡阳,421001 3. 南华大学生命科学与技术学院 |
| 摘 要： | 目的建立红鲫C1HD近交系的RAPD标记。方法从80条随机引物中筛选出20条扩增效果和多态性较好的引物,对8尾红鲫C1HD近交系和8尾普通红鲫基因组DNA进行RAPD扩增。结果S333引物扩增出一条特异性条带,大小约为2.1 kb。结论S333引物扩增出的特异性条带可以作为区分普通红鲫与红鲫C1HD近交系的分子遗传标记。 |
| 关 键 词： | 近交系 红鲫 分子标记 |
| 文章编号： | 1671-7856（2007）10-0589-03 |
| 修稿时间： | 2007-03-29 |
Bolting of RAPD Marker on C1HD Inbred Red Crucian Carp,Carrassius auratus Red Variety |
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| TIAN Zhi,WU Duan-sheng,ZHEN Ji-fang,JIANG Qian-qiu.Bolting of RAPD Marker on C1HD Inbred Red Crucian Carp,Carrassius auratus Red Variety[J].Chinese Journal of Comparative Medicine,2007,17(10):589-591. | |
| Authors: | TIAN Zhi WU Duan-sheng ZHEN Ji-fang JIANG Qian-qiu |
| Institution: | 1. College of Life Science and Technology of University of South China; 2. Department of Laboratory Animal Science of University of South China, Hengyang 421001, China; 3 .Changsha Medical College, Changsha 410219, China |
| Abstract: | Objective To bolt RAPD marker from Carassius auratus red variety inbred strain C1HD.Methods Using 20 random primers from 80 primers,8 samples of inbred strain C1HD and common Carassius auratus red variety were analyzed by random amplified polymorphic DNA(RAPD),respectively.Results A primer(S333) was observed to produce a specific band.Conclusion This band could be used as a molecular marker for discriminating Carassius auratus red variety inbred strain C1HD from common population. |
| Keywords: | RAPD |
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