体外培养的人睾丸间质细胞对绒毛膜促性腺激素刺激的反应

目的：探讨体外培养的人睾九间质(Leydig)细胞对绒毛膜促性腺激素(hCG)刺激的反应。方法：随机选择人离体睾九20份进行Leydig细胞原代及传代培养，同一人份Leydig细胞分成4组，不同时间用hCG刺激，连续收集培养上清液，用磁性分离免疫酶联测定法测定睾酮含量。结果：原代细胞对首次hCG的刺激反应敏感，连续刺激则睾酮的分泌受抑制，间隔2天再次刺激时虽有反应，但明显减弱。传代后的Leydig细胞对hCG的刺激几乎无反应。结论：原代Leydig细胞对hCG刺激的反应受刺激时间、刺激次数的影响，临床使用hCG治疗时应注意。

The Stimulation Effects of hCG on the Human Leydig Cells Cultured in Vitro

Objective: To study the stimulation effects of hCG on the Leydig cells cultured in vitro. Methods: Leydig cells were randomly selected from isolated human testis to primarily culture and subcultur. The same sample was divided into 4 groups. The Ley-dig cells were stimulated with hCG on different periods and the culture fluids collected to examinate the testoterone by SEROZYME system. Results: The primarily cultured and subcultured Leydig cells were sensitive to hCG in first stimulation but the secretion of testoterone was inhibited by continuous stimulation. After 2 days the secretion of testoterone was lower by second time stimulation with hCG. Subculure Leydig cells hardly responded to hCG. Conclusion: The response of primarily cultured Leydig cells to hCG may be related to the duration and times of stimulation. The treatment with hCG should raise wide concern about its stimulation.