寒湿痹片抑制大鼠滑膜增生及相关机制研究

目的:观察寒湿痹片(HSB)对灭活结核分枝杆菌(heat-killed mycobacterium tuberculosis H37Ra.Mtb)诱导SD大鼠类风湿关节炎(Adjuvant-induced rheumatoid arthritis.AA)滑膜损伤的抑制作用及对基质金属蛋白酶MMP-1,MMP-3mRNA表达影响。方法:以Mtb免疫SD大鼠,制备实验性类风湿关节炎模型。采用核酸原位杂交法检测类风湿关节炎大鼠关节滑膜组织中MMP-1、MMP-3mRNA的表达。结果:寒湿痹片对Mtb诱导SD大鼠类风湿关节炎踝关节滑膜损伤具有显著的抑制作用,并减少MMP-1、MMP-3mRNA在滑膜组织中的表达。结论:寒湿痹片抑制实验性类风湿关节炎滑膜损伤,这种作用与减少滑膜组织MMP-1,MMP-3mRNA表达相关。

Effects of Hanshibi Pellet on Synovial Membrane Hyperplasia and Study on Related Mechanism

Objective:To study the effects of Hanshibi Pellet(HSB) on synovial membrane hyperplasia and expression of MMP-1 and MMP-3 mRNA in rheumatiod arthritis induced by heat-killed mycobacterium tuberculosis H37Ra(Mtb) in SD rats.Methods: The adjuvant-induced rheumatoid arthritis(AA) in SD rats was established by sc immunization with the heat-killed mycobacterium tuberculosis H37Ra(Mtb).The expression of MMP-1 and MMP-3 mRNA in synovial membrane was measured by using nucleic acid hybridization in situ.Results:HSB could significantly inhibit the injury in ankle joint and decrease the expression of MMP-1 and MMP-3 mRNA in synovial membrane of AA model rats.Conclusions: HSB can inhibit the injury in ankle joint,which is concerned with the decrease of MMP-1 and MMP-3 mRNA expression in synovial membrane of AA model rats.