| 急性白血病骨髓单个核细胞糖基化磷脂酰肌醇特异性磷脂酶D的表达(英文) |
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| 引用本文： | 肖广芬,陈方平,王光平,付斌,谢俊明,程英妮,李群,蹇在伏.急性白血病骨髓单个核细胞糖基化磷脂酰肌醇特异性磷脂酶D的表达(英文)[J].中国组织工程研究与临床康复,2010,14(6). |
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| 作者姓名： | 肖广芬 陈方平 王光平 付斌 谢俊明 程英妮 李群 蹇在伏 |
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| 作者单位： | 1. 中南大学湘雅三医院血液科,湖南省长沙市,410013
2. 中南大学湘雅医院血液科,湖南省长沙市,410008 |
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| 摘 要： | 背景:据作者查新检索,国内外有关急性白血病骨髓单个核细胞糖基化磷脂酰肌醇特异性磷脂酶D活性及mRNA表达与白血病类型、患者肝脾淋巴结肿大关系的报道罕见.目的:探讨糖基化磷脂酰肌醇特异性磷脂酶D的表达与急性白血病类型、急性髓系白血病患者肝脾淋巴结肿大的关系.方法:急性髓系白血病组骨髓标本43例,急性淋巴细胞白血病组骨髓标本28例,以21例健康志愿者作为正常对照组.密度梯度离心法获得骨髓单个核细胞,用GPI锚定的胎盘碱性磷酸酶作为底物,Triton-X114分相法检测糖基化磷脂酰肌醇特异性磷脂酶D的活性,半定量RT-PCR法检测糖基化磷脂酰肌醇特异性磷脂酶D mRNA的表达,并分析糖基化磷脂酰肌醇特异性磷脂酶D活性和mRNA表达水平与急性白血病类型、急性髓系白血病患者肝脾淋巴结肿大的关系.结果与结论:与正常对照组比较,急性髓系白血病组骨髓单个核细胞糖基化磷脂酰肌醇特异性磷脂酶D活性和mRNA表达均明显升高(P < 0.01);急性淋巴细胞白血病组骨髓单个核细胞糖基化磷脂酰肌醇特异性磷脂酶D活性和mRNA表达均明显降低(P < 0.01).在43例急性髓系白血病患者中,13例患者检查发现有肝脾淋巴结肿大,30例患者检查无肝脾淋巴结肿大,无肝脾淋巴结肿大标本骨髓单个核细胞糖基化磷脂酰肌醇特异性磷脂酶D活性和mRNA表达均明显高于有肝脾淋巴结肿大标本(P < 0.05).结果证实急性白血病骨髓单个核细胞糖基化磷脂酰肌醇特异性磷脂酶D的活性与mRNA变化一致,其表达水平与急性白血病类型及急性髓系白血病患者有无肝脾和/或淋巴结肿大有关.
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| 关 键 词： | 白血病 糖基化磷脂酰肌醇特异性磷脂酶D 髓外浸润 骨髓单个核细胞 干细胞 |
Glycosylphosphatidilinoditol-specific phospholipase D expression in bone marrow mononuclear cells derived from acute leukemia patients |
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| Xiao Guang-fen,Chen Fang-ping,Wang Guang-ping,Fu Bin,Xie Jun-ming,Cheng Ying-ni,Li Qun,Jian Zai-fu.Glycosylphosphatidilinoditol-specific phospholipase D expression in bone marrow mononuclear cells derived from acute leukemia patients[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2010,14(6). |
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| Authors: | Xiao Guang-fen Chen Fang-ping Wang Guang-ping Fu Bin Xie Jun-ming Cheng Ying-ni Li Qun Jian Zai-fu |
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| Institution: | Xiao Guang-fen1,Chen Fang-ping2,Wang Guang-ping2,Fu Bin2,Xie Jun-ming2,Cheng Ying-ni2,Li Qun2,Jian Zai-fu2 1Department of Hematology,Third Xiangya Hospital,Central South University,Changsha 410013,Hunan Province,China,2Department of Hematology,Xiangya Hospital,Changsha 410008 |
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| Abstract: | BACKGROUND:The correlation of gycosylphosphatidilinoditol-specific phospholipase D (GPI-PLD) activity,mRNA expression to leukemia type,hepatosplenomegaly and/or lymphadenopathy has been rarely reported.OBJECTIVE:To explore the correlation of GPI-PLD expression to leukemia type and hepatosplenomegaly and/or lymphadenopathy of acute myeloid leukemia (AML) patients.METHODS:Fresh bone marrow specimens were obtained from 43 newly diagnosed AML patients,28 acute lymphocytic leukemia (ALL) patients,and 21 normal p... |
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