经皮睾丸精子抽吸术结合卵浆内单精子注射治疗严重男性因素不育的研究

目的 了解经皮睾丸精子抽吸术（PTSA）结合卵浆内单精子注射（ICSI）技术治疗严重男性因素不育的治疗效果。方法 于1998年10月10日至2000年12月31日,我中心采用PTSA结合ICSI技术治疗162对严重男性因素不育的夫妇,短方案超排卵,对处于细胞分裂中期（MⅡ期）的成熟卵母细胞行ICSI。结果 185个周期共注射1517个MⅡ期卵母细胞,其中990个正常受精,正常受精率为65.3%,移植胚胎周期152个,54例妊娠,临床妊娠率为35.5%。结论 PTSA结合ICST技术为严重男性不育症患者提供了一种快速、方便、无痛、有效的治疗方法。

Percutaneous testicular sperm aspiration and intracytoplasmic sperm injection in the treatment of severe male infertility

OBJECTIVE: To investigate the efficacy of intracytoplasmic sperm injection (ICSI) combined with percutaneous testicular sperm aspiration (PTSA) in the treatment of severe male infertility. METHODS: From October 1998 to December 2000, 162 couples were enrolled, and ICSI and PTSA were adopted. Ovarian stimulation was achieved by the short protocol. All metaphase II (M II) oocytes were selected for ICSI. RESULTS: 1,517 M II oocytes were injected in 185 cycles, 990 fertilized (65.3%), and 152 embryo implanted. 54 couples achieved clinical pregnancy (35.5%). CONCLUSION: PTSA combined with ICSI is a rapid, convenient, painless and effective approach for the treatment of severe male infertility.