低氧增高小鼠脑组织内JNK1的磷酸化水平

目的 观察应激激活的蛋白激酶(JNK/SAPK)磷酸化水平在小鼠脑低氧预适应过程中的变化。方法 利用我室已建立的小鼠低氧预适应模型,制备重复低氧1～4次小鼠(H1～H4),结合应用SDS—聚丙烯酰胺凝胶蛋白电泳(SDS—PAGE)、蛋白印迹(Western bolt)等生化技术,半定量检测海马和大脑皮层内JNK的磷酸化水平。结果 小鼠海马组织内JNK1的磷酸化水平随着低氧次数(H1～H4)的增加而明显增高,且与正常对照组(H0)相比具有统计学上的显意义(P<0.05);而对于大脑皮层组织内JNK1磷酸化水平的增高,则只在低氧1、2次(H1和H2)组有在统计学上具有显著意义(P<0.05);此外,在小鼠脑组织内,尤其是海马组织内未检测到磷酸化JNK2/3。结论 JNK1可能在脑低氧预适应过程中具有重要作用。同时,海马和大脑皮层组织内JNK1磷酸化水平对重复性低氧刺激反应的不一致可能与它们对低氧耐受不同有关。

Influence of Hypoxia on JNKI Phosphorylation Level in the Brain of Mice

Objective To observe the changes of C -Jun NH2 -terminal kinase or stress -activated protein kinase (JNK/SAPK) phosphorylation level in the brain during the development of cerebral hypoxic pre-adaptation. Methods By using the model of hypoxic pre-adaptation mice, which was established before in our lab, the repetitive hypoxic mice (H1 -H4) were prepared, and JNK phosphorylation level in hippocampus and cerebral cortex was determined quantitatively by SDS-PAGE, Western blot and Gel Doc system. Results It was found that JNK1 phosphorylation level was significantly ( P < 0.05) increased in response to repetitive hypoxia (HI -H4) in the hippocampus, but the increments of JNK1 phosphorylation level in cerebral cortex of mice were significant ( P <0. 05) only in the group of H1 and H2. But the phosphorylated JNK2/3 in the brain, especially in the hippocampus were not detected. Conclusions The results suggested that JNK1 not JNK2/3 might play an important role in the development of cerebral hypoxic pre-adaptation and the different responseofJNKl phosphorylation inhippo-campus and cerebral cortex of micetorepetitivehypoxia could be related to theirtolerancetohypoxia.