川芎配方颗粒提取工艺及咖啡酸、阿魏酸含量测定

目的：优选川芎配方颗粒提取工艺,建立咖啡酸和阿魏酸含量的测定方法.方法：以正交试验优选川芎配方颗粒的提取工艺,采用HPLC建立其咖啡酸、阿魏酸含量测定方法.结果：优选出川芎配方颗粒提取工艺为10倍量水,浸泡0.5h,每次1h,提取3次;咖啡酸和阿魏酸HPLC测定方法为：流动相：乙腈-0.1％磷酸（14∶86）;检测波长：323nm;流速：1.0mL/min,理论板数按阿魏酸峰计算应不低于4 000.结论：该提取工艺有效成分提取效率高,质量控制方法稳定可行,可作为川芎配方颗粒生产和质量控制的科学依据.

Extraction Process and Content Determination of Coffee Acid and FeruLic Acid in Chuanxiong FormuLa Granule

Objective： Optimization of extraction process of Chuanxiong formula granule, establish Coffee acid and method to deter mine the content of ferulic acid. Methods ： The optimum extraction process of Chuanxiong formula granule by orthogonal test, using HPLC to establish a method for determination of the Coffee acid, ferulic acid. Results：To optimize the extraction process of Chuanxiong formula granule was 10 times the volume of water, soak 0.5h, lh each time, extracting 3 times; for the determination of Coffee acid and ferulic acid in mobile phase acetonitrile HPLC： -0.1 % phosphate （14：86）; the detection wavelength： 323nm; flow rate： 1.0mL/min, according to the number of board of ferulic acid peak calculation should not be less than 4 000. Conclusion： The effective components of the extraction efficiency is high, the quality control method is stable and feasible, and can be used as a scientific basis for Chuanxiong formula granule production and quality control.