| HPLC-ELSD法测定胆南星中胆酸含量 |
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| 引用本文： | 王光函,;姜鸿,;邹桂欣,;尤献民.HPLC-ELSD法测定胆南星中胆酸含量[J].亚太传统医药,2014(22):8-9. |
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| 作者姓名： | 王光函 ;姜鸿 ;邹桂欣 ;尤献民 |
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| 作者单位： | [1] 辽宁省中医药研究院,辽宁沈阳110034; [2]辽宁中医药大学,辽宁大连116600 |
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| 基金项目： | 国家科技部重大新药创制项目（2012ZX09102201-005） |
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| 摘 要： | 目的：建立HPLC-ELSD法测定胆南星中胆酸含量的方法.方法：色谱柱为Hypersil BDS C18色谱柱（4.6mm×200mm,5μm）,流动相为乙腈—水（36∶64）,柱温为25℃,流速为1.0mL·min1.检测器为ELSD,漂移管温度为100℃,载气流量为3.0L·min-1.结果：胆酸在0.14～1.4μg进样量范围内与其峰面积积分呈良好的线性关系（r=0.999 4）,平均加样回收率为97.13％,RSD=1.80％.结论：该方法简便准确,灵敏度高,可用于胆南星中胆酸含量的测定和产品质量控制.
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| 关 键 词： | 胆南星 HPLC—ELSD 胆酸 |
Determination of Cholic acids in Bile Arisaema by HPLC-ELSD |
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| Institution: | Wang Guanghan ,Jiang Hong ,Zou Guixin ,You Xianmin(1. Liaoning Provincial Institute of Traditional Chinese Medicine, Shenyang 110034, China ; 2. Liaoning University of Traditional Chinese Medicine,Dalian 116600, China) |
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| Abstract: | Objective：To develop a HPLC method for the determination of cholic acids in Bile Arisaema. Methods： Hypersil BDS C18 column （4.6mm×200mm, 5μm）in an oven at 25℃ was need, with a mobile phase consisting of acetonitrile-water（36 ： 64）, the flow rate was 1.0mL · min-1. The drift tube temperature of ELSD was 100℃ ,and the flow rate of air was 3.0L ·min-1. Results： The linear ranges of cholic acids was 0.14～ 1.4μg（r= 0. 999 4）, the average recovery was 97. 13%, RSD= 1.80%. Conclusion： The method was simple and accuate, and can be used for the quality control of Bile Arisaema. |
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| Keywords: | Bile Arisaema HPLC-ELSD Cholic Acids |
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