| 蒽酮-硫酸分光光度法测定五味子中总多糖含量 |
| |
| 引用本文： | 徐小娜,;鲁岐,;宋凤瑞,;黄海龙. 蒽酮-硫酸分光光度法测定五味子中总多糖含量[J]. 亚太传统医药, 2014, 0(15): 15-17 |
| |
| 作者姓名： | 徐小娜, 鲁岐, 宋凤瑞, 黄海龙 |
| |
| 作者单位： | [1]南华大学,湖南衡阳421001; [2]大连富生天然药物开发有限公司,辽宁大连116600; [3]中国科学院长春应用化学研究所,吉林长春130022 |
| |
| 基金项目： | 国家自然科学基金项目（11205085）;湖南省教育厅项目（12C0334）;衡阳市科技局项目（2013KJ14,2013KJ15） |
| |
| 摘 要： | 通过单因素实验选择和正交设计对五味子中多糖进行超声辅助提取,以吸光度值为指标,分别对料液比、提取温度、提取时间和提取次数等因素进行考察,优化出超声辅助提取五味子多糖的最佳条件为料液比1∶30、提取时间40min、温度60℃、提取次数为1次.在优化的条件下对湖北和辽宁产的五味子中总多糖含量进行测定,得出北五味子总多糖含量为39.31mg/g,南五味子总多糖含量为24.05mg/g,二者多糖含量存在显著性差异.
|
| 关 键 词： | 五味子 蒽酮-硫酸 多糖 含量测定 |
Determination of Total Polysaccharide in Schisandra Chinensis Turcz. Bail by Anthrone-sulfuric Acid Spectrophotometric Method |
| |
| Affiliation: | Xu Xiaona , Lu Qi , Song Fengrui , Huang Hailong (1. School of Public Health, University of South China, Hengyang 421001,China2. Dalian Fusheng natural drug development Co. Ltd. , Dalian 116600,China 3. Changchun Institute of Applied Chemistry,Chinese Academy of Sciences, Changchun 130022, China) |
| |
| Abstract: | In this study, single factor and orthogonal design was used for ultrasonic assisted extraction of polysaccharide fromSchisandra chinensis Turcz. Bail. Based on absorbance value, the solid-liquid ratio, extraction temperature, extraction time and ex-traction frequencies and other factors were investigated. The best conditions for the ultrasonic method to extract polysaccharide wereas follows., solid-liquid ratio（m/V） was lg ： 30mL, extraction temperature was set at 60℃, extraction time was 40min, extractingfor 1 time. Under this condition, the polysaccharide of Schisandra chinensis Turcz. Bail. from Hubei and Liaoning province were re-spectively detected. The results obtained show there are significant differences between the tested samples on the content of polysac-charide.Key Words ： Schisandra Chinensis Turcz. Bail; Anthrone-sulfuric Acid; Polysaccharide |
| |
| Keywords: | Schisandra Chinensis Turcz. Bail Anthrone-sulfuric Acidl Polysaccharide |
| 本文献已被 维普 等数据库收录！ |
|
