结直肠癌患者手术前后肠道菌群变化分析

为通过观察结直肠癌患者手术前后肠道中双歧杆菌（Bifidobacteriumspp．）、乳酸杆菌（Lactobacillus）、粪肠球菌（Enterococcus如ecalis）和拟杆菌（Bacteroidaceae）的变化情况，分析肠道菌群的改变在结直肠癌发病及治疗中的作用及意义，收集正常健康人粪便标本60份、结直肠癌患者术前及术后粪便标本各60份，应用平板活菌计数法和荧光定量聚合酶链反应（PCR）法检测粪便标本中各种菌群的数量，同时采集患者的外周血标本，应用显色法检测D-乳糖和内毒素的含量。结果显示，结直肠癌患者术前粪便中乳酸杆菌、双歧杆菌数量均比正常健康人少，P〈O．05或P〈O．01；粪肠球菌、拟杆菌数量均比正常健康人多，P〈O．05或P〈O．01。结肠癌患者术后粪便中乳酸杆菌、双歧杆菌数量进一步减少，P〈0．05或P〈0．01；粪肠球菌、拟杆菌数量进一步增多，P〈O．05或P〈O．01。结直肠癌患者血清I）I乳糖、内毒素含量比正常健康人高，P〈O．Ol，手术后其含量进一步升高，P〈O．Ol。结果表明，肠道菌群的变化与结直肠癌的发生、发展及预后有一定关系。

Analysis on the Enteric Bacterium Flora Change of Patients before and after Surgery for Colorectal Carcinoma

Through observation on the change status of the enteric bacterium flora such as Bifidobacteriurn spp. , Lactobacillus , Enterococcus faecalis and Bacteroidaceae this study was to analyzed the action and significance of enteric bacterium flora change in the pathogenesis and treatment of co｜orectal carcinoma. Authors collected 60 feces specimen from health person, 60 preoperative feces specimen and 60 postopera= tive feces specimen from colorectal carcinoma patients, by using plate-mehtod for counting viable bacterium and fluorescent quantitative polymerase chain reaction（PCR） detected the contents of each flora of those specimen;at this time, collected the peripheral blood specimen of patients, by using chromogenie test detec- ted the contents of D-lactose and endotoxin. As results, the contents of Lactobacillus and Bifidobacterium sppl from fecel specimen of patients before surgery were lower than that of health person（ P 〈0.05 or P 〈0.01） ,also further decreased after operation（ P 〈0.05 or P 〈0.01）, but the contents of Enterococcus faecalis and Bateroidaceae higher（ P 〈0. 05 or P 〈0.01） ~also further increased（ P 〈0. 05 or P 〈0. 01） ; the contents of peripheral serum D-lactose and endotoxin in patients were higher than that in health person （ P 〈0.01） ,also further increased after operation（ P 〈0.01）. Results show that the change of enteric bac- terium flora is related tO the pathogenesis,develol0rnent and prognosis of colorectM cancer certainly.