中国南方食管鳞癌D5S107和D5S408杂合性丢失与微卫星不稳定性

 【目的】研究中国南方食管癌高发地区（潮汕地区）食管鳞癌5号染色体长臂（5q）部分微卫星标记的杂合性丢失（LOH）和微卫星不稳定（MSI），为食管鳞癌相关抑癌基因的定位提供依据。【方法】采用PCR银染技术，检测58例配对食管鳞状细胞癌标本多个微卫星标记（D5S107；SHGS31088；D5S816；D5S625和D5S408）的杂合性丢失和微卫星不稳定。【结果】SHGS31088，D5S816和D5S625杂合性丢失率和微卫星不稳定都较低；而D5S107和D5S408的杂合性丢失率分别为48.5%和34.3％，微卫星不稳定率分别为9.1％和5.7％。【结论】中国南方食管鳞癌D5S107和D5S408的杂合性丢失率较高，提示在D5S107和D5S408附近可能存在食管癌相关基因。5q区域5个微卫星标记的微卫星不稳定发生率都较低，提示微卫星不稳定在中国南方食管癌发生过程中可能不占主导地位。

Loss of Heterozygosity and Microsatellite Instability of Esophageal Squmous Cell Carcinoma in South China

[Objective] To study the loss of heterozygosity (LOH) and microsatellite instability (MSI) with some microsatellite markers on 5q of esophageal squmous cell carcinoma (ESCC) in high incidence area of South China (Chaoshan area). It will provide scientific basis for looking for the position(s) of candidate tumor suppressor gene(s) on 5q in ESCC.[Methods] Polymerase chain reaction (PCR) based on LOH and MSI was performed in 58 primary ESCC with 5 microsatellite markers(D5S107, SHGS31088, D5S816, D5S625, and D5S408).[Results]Both of the frequencies of LOH and MSI were low at SHGS31088, D5S816, and D5S625. The frequencies of LOH at D5S107 and D5S408 were 48.5% and 34.3%, respectively. The frequencies of MSI at D5S107 and D5S408 were 9.1% and 5.7%, respectively. [Conclusions]According to the high frequencies of LOH at D5S107 and D5S408, there probably existed tumor suppressor gene(s) around D5S107 and D5S408 in ESCC of South China. On the other hand, the frequencies of MSI at 5 microsatellite markers on 5q were very low, indicating MSI might not be the most important reason for the occurrence in ESCC of South China.