Quantitative analysis of serum lipoproteins by micro-scale thin-layer chromatography. |
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| Authors: | G L Mills C E Taylaur A L Miller |
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| Affiliation: | 1. Laboratory of Biochemistry, B.C.P. Jansen Institute, University of Amsterdam, Plantage Muidergracht 12, 1018 TV AmsterdamThe Netherlands;2. Amsterdam Centre for Rheumatic Diseases, Dr. J. van Breemenstraat 2, 1056 AB AmsterdamThe Netherlands |
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| Abstract: | A method has been devised for the complete chemical analysis of serum lipoproteins, in which the constituents are separated by thin-layer chromatography and then measured by means of a flame ionisation detector. Since the response of the detector differs for each constituent, it is necessary to use a previously prepared calibration curve for each one. A complete analysis can be obtained from a single run on about 20 microgram of lipoprotein. However, from 5--10 chromatograms are needed for an adequate degree of precision. The method, which could be adapted to the measurement of tissue lipids, takes less than 2 h to complete. This speed and simplicity seem to give the method considerable potential for the investigation of patients with disorders of lipid transport. |
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| Keywords: | To whom correspondence should be addressed. |
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