Characterization and Application of a Lytic Phage D10 against Multidrug-Resistant Salmonella |
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| Authors: | Zhiwei Li Wanning Li Wenjuan Ma Yifeng Ding Yu Zhang Qile Yang Jia Wang Xiaohong Wang |
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| Affiliation: | 1.College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; (Z.L.); (W.L.); (W.M.); (Y.D.); (Y.Z.); (Q.Y.); (J.W.);2.Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China |
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| Abstract: | Salmonella is a widely distributed foodborne pathogen that is a serious threat to human health. The accelerated development of drug resistance and the increased demand for natural foods invoke new biocontrol agents to limit contamination by multidrug-resistant (MDR) Salmonella strains. In this study, a lytic Salmonella phage named D10 was characterized at the biological and genomic levels. D10 possesses a short latent period (10 min) and a large burst size (163 PFU/cell), as well as adequate stability under a range of pH conditions and moderate thermal tolerance. D10 effectively lysed different MDR Salmonella serovars and repressed their dynamic growth in the medium. Genomic analysis disclosed that D10 is a new member of the Siphoviridae family and lacks the genes implicated in lysogeny, pathogenicity, or antibiotic resistance. A three-ingredient phage cocktail was then developed by mixing D10 with previously identified myovirus D1-2 and podovirus Pu20. The cocktail significantly reduced the count of MDR strains in liquid eggs, regardless of the temperature applied (4 and 25 °C). These results suggest that phage D10 is a promising tool to prevent food contamination by MDR Salmonella. |
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| Keywords: | Salmonella multidrug-resistant phage genomic cocktail lysis eggs |
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