| 利用流式细胞术研究榄香烯对肝癌腹水瘤细胞系Hca—F25／CL—1 … |
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| 引用本文： | 左云飞,郑军. 利用流式细胞术研究榄香烯对肝癌腹水瘤细胞系Hca—F25／CL—1 …[J]. 大连医科大学学报, 2000, 22(1): 46-49 |
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| 作者姓名： | 左云飞 郑军 |
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| 作者单位： | 大连医科大学检验系!116027(左云飞，张耀铮)，大连医科大学检验系!94级(郑军) |
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| 摘 要： | 采用小鼠肝癌腹水瘤细胞系Ｈｃａ－Ｆ２５／ＣＬ－１６Ａ３，利用流式细胞术研究了榄香烯对肿瘤细胞的作用，对Ｈｃａ－Ｆ２５／Ｃｌ－１６Ａ３细胞周期的影响、ＤＮＡ含量的分析以诱导细胞凋亡。结果表明：榄香烯可以明显抑制Ｈｃａ－Ｆ２５／ＣＬ－１６Ａ３细胞的增殖，有效地杀伤肿瘤细胞；榄香烯对ＣＯ／ＧＩ期细胞无明显作用，主要作用于Ｓ期，阻止由Ｓ期进入Ｇ２／Ｍ期，从而抑制肿瘤生长；可以ＧＯ／ＧＩ期前出现一个凋亡特异
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| 关 键 词： | 流式细胞术 榄香烯 凋亡 肝癌 腹水 |
| 文章编号： | 1000-5676(2000)01-0046-04 |
| 修稿时间： | 1999-07-13 |
The study of the antitumor activity of elemene by flow cytometry(FCM) |
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| Zuo Yunfei,Zhang Yaozheng,Zheng Yun [WTBX]. The study of the antitumor activity of elemene by flow cytometry(FCM)[J]. Journal of Dalian Medical University, 2000, 22(1): 46-49 |
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| Authors: | Zuo Yunfei Zhang Yaozheng Zheng Yun [WTBX] |
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| Affiliation: | Zuo Yunfei,Zhang Yaozheng,Zheng Yun(Department of Laboratory, Dalian Medical University, Dalian , China) |
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| Abstract: | In the review,author analyzed the antitumor activity of elemene preparation,influence for Hca F25/CL 16A3,DNA content and the function with induction apoptosis with Hca F25/CL 16A3 by flow cytometry(FCM).Result:Elemene could inhibit the growth of tumor abviously,kill the tumor cell.During period of the reaction,GO/G1phase did not change,elemene could arrest cell cycle from S phase to G2/M phase transtion and make it more in S phase.At last,inhibit the tumor cell,and become a AP phase in front of GO/G1phase. [WT5HZ] |
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| Keywords: | flow cytometry elemene apoptosis |
| 本文献已被 CNKI 维普 等数据库收录！ |
