铁包金中不同部位总黄酮含量比较

目的:考察了贵州都匀产铁包金不同部位总黄酮含量的差异.方法:以乙醇作为回流提取溶剂提取铁包金中总黄酮.以芦丁为对照品,采用紫外分光光度法测定铁包金中的总黄酮.结果:芦丁标准品溶液浓度在5-30 μg· L-1与吸光度有良好线性关系,回归方程为Y=0.011 2X+0.041 9(r =0.999 1),平均加样回收率98.56％,精密度试验RSD值为0.279％,重复性试验RSD为0.634％,稳定性试验RSD 1.49％.以此方法测定,铁包金叶和茎部中黄酮含量分别为12.3％,1.42％,叶中总黄酮含量是茎部的的8.7倍.结论:贵州都匀产铁包金叶和茎部中黄酮含量存在较大差异,叶部位具有较大的药用开发价值和应用前景.

Comparison and Determination of Total Flavonoids in Different Parts of Berchemia lineata

Objective: The content distribution of total flavonoids in different parts of Berchemia lineate from city of DuYun in Guizhou was investigated.Method:Flavonids in different parts of Berchemia Lineate(L)DC.were extracted by ethanol as solvent reflux,The total flavonoids were determined by spectrophotometry,rutin as control sample.Result:The absorbency and concentration of rutin have good linear correlation in the range of 5-30 μg·L-1.The regression equation was Y=0.011 2X+0.041 9(r=0.999 1),the average recovery rate was 98.56%,Accuracy experiment RSD 0.279%,repeatability test RSD 0.634%,stability test RSD 1.49%,the contents of total flavonoids in leaf and stem was respectively 12.30% and 1.42%,that the means content of total flavonoids in leaf is 8.7 times higher than that in stem.Conclusion: the content of total flavonoids in the leaf and in the stem has a big difference,thereby the leaf of B.lineate from Duyun in Guizhou has value to be developed and used.