柴胡疏肝散不同配伍对柴胡皂苷a溶出量的影响

目的:研究柴胡疏肝散不同配伍对柴胡皂苷a溶出量的影响.方法:采用L8(27)正交设计法,以HPLC-DAD测定各配伍样品中柴胡皂苷a的含量,并对数据进行单因素方差分析.结果:正交设计各因素对柴胡皂苷a溶出率的影响大小为使药＞佐药＞臣药.单因素对其影响均有显著性差异,交互作用无显著性差异.4组分法中,各组间均有显著性差异.不同药味数分组中,各大组之内不同配伍柴胡皂苷a溶出率不同,各组间溶出率:复方＞五五配伍＞单煎液＞六六配伍、两两配伍＞四四配伍＞三三配伍.结论:含有白芍、香附、川芎的配伍组中柴胡皂苷a的溶出率较低,含有枳壳和甘草配伍组中柴胡皂苷a的溶出率较高,全方组中柴胡皂苷a的溶出率最高.

Influence of Different Compatibility of Chaihu Shugan Powder on Dissolution of Saikosaponin a

Objective: To study on influence of different compatibility of Chaihu Shugan powder on dissolution of saikosaponin a. Method: L₈(2⁷) orthogonal design method was used with determining the content of saikosaponin a in each compatibility samples by HPLC/DAD,and data was analyzed by single-factor variance analysis. Result: Influence degree of every factors in orthogonal test on dissolution of saikosaponin a was:guide> adjuvant> associate. Effect of it(dissolution of saikosaponin a) had significant difference by single-factor, but there was no significant difference in interaction. It had significant difference among groups in the 4 groups classified.In the different number of drugs classified,dissolution of saikosaponin a was different in every major groups with different compatibility, dissolution rates among every groups:the compound group> five-five compatibility group> single-decoction group> six-six compatibility group, two-two compatibility group> four-four compatibility group> three-three compatibility group. Conclusion: Dissolution of saikosaponin a was lower from groups including Radix Paeoniae Alba, Rhizoma Cyperi and Rhizoma Chuanxiong, it was higher from groups including Auranti Fructus and Glycyrrhiza Uralensis, it was the highest from the compound group.