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糖尿病视网膜病变早期血管内皮生长因子作用机制的研究
引用本文:宋鄂,董宇,王浩东,徐琪,王心蕊,吴家祥. 糖尿病视网膜病变早期血管内皮生长因子作用机制的研究[J]. 中国病理生理杂志, 2004, 20(8): 1440-1443
作者姓名:宋鄂  董宇  王浩东  徐琪  王心蕊  吴家祥
作者单位:1. 吉林大学第一医院眼科, 吉林 长春 130021;
2. 吉林大学医疗系, 吉林 长春 130021;
3. 吉林大学基础医学部生理学教研室, 吉林 长春 130021;
4. 吉林大学基础医学部病理解剖学教研室, 吉林 长春 130021
基金项目:吉林省科学基金资助项目 (No .990 5 75 - 5 ),吉林大学新技术新疗法基金资助项目 (No.2 0 0 10 9)
摘    要:目的:通过链脲佐菌素(streptozocin,STZ)糖尿病大鼠视网膜的石蜡切片及血管铺片,研究糖尿病大鼠视网膜病变时血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)作用的分子病理机制。方法:建立糖尿病大鼠模型,随机分为正常对照组(C),糖尿病1月组(M1)、3月组(M3)、5月组(M5)。分别在视网膜石蜡切片及血管铺片上行VEGF原位杂交和免疫组化。结果:①石蜡切片:原位杂交仅M5表达为67%;免疫组化M3表达为34%,M5表达为89%。②视网膜血管铺片:原位杂交仅M5表达为34%;免疫组化仅M5表达为56%。结论:①除内皮细胞外,周细胞及Müller细胞也可产生VEGF;②糖尿病视网膜病变早期VEGF可能是来源于旁分泌途径。

关 键 词:糖尿病视网膜病变  免疫组织化学  原位杂交  内皮生长因子  
文章编号:1000-4718(2004)08-1440-04
收稿时间:2003-05-16
修稿时间:2003-05-16

Mechanisms of vascular endothelial growth factor in diabetic rat retinopathy
SONG E,DONG Yu,WANG Hao-dong,XU Qi,WANG Xin-rui,WU Jia-xiang. Mechanisms of vascular endothelial growth factor in diabetic rat retinopathy[J]. Chinese Journal of Pathophysiology, 2004, 20(8): 1440-1443
Authors:SONG E  DONG Yu  WANG Hao-dong  XU Qi  WANG Xin-rui  WU Jia-xiang
Affiliation:1. Department of Ophthalmology, The First Hospital, School of Basic Medical Sciences, Jilin University, Changchun 130021, China;
2. Department of Medicine, School of Basic Medical Sciences, Jilin University, Changchun 130021, China;
3. Department of Physiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China;
4. Department of Pathology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China
Abstract:AIM: To study the molecular mechanism of vascular endothelial growth factor (VEGF) in pathogenesis of diabetes in rats. METHODS: The diabetic rat model was established by using streptozocin. The animals were divided into normal control (C group), diabetic for one month (M1 group), for three months (M3 group) and for five months (M5 group). In situ hybridization and immunohistochemistry were conduced to observe the expression of VEGF on retinal digest preparation and paraffin section. RESULTS: 1. On paraffin section: the positive rate of VEGF expression in M5 group was 67% by in situ hybridization and 89% by immunohistochemistry. There was only 34% positive expression of VEGF in M3 group by immunohistochemistry. 2. On retinal digest preparation: the VEGF positive expression rate in M5 group was 34% by in situ hybridization and 56% by immunohistochemistry. CONCLUSION: Both endothelial cells and Mural cells and Müller cells express VEGF. The source of VEGF may be from the paracrine pathway in early stage of diabetic retinopathy.
Keywords:Diabetic retinopathy  Immunohistochemistry  In situ hybridization  Endothelial growth factor
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