| WNK4基因与原发性高血压的相关性分析 |
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| 引用本文： | Sun ZJ,Wang XN,Lu JY,Ding Q,Dong LY,Zhao YY. WNK4基因与原发性高血压的相关性分析[J]. 中国医学科学院学报, 2003, 25(2): 145-148 |
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| 作者姓名： | Sun ZJ Wang XN Lu JY Ding Q Dong LY Zhao YY |
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| 作者单位： | 中国医科大学医学遗传学教研室,沈阳,110001 |
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| 基金项目： | 国家自然科学基金(39970411),教育部高等学校骨干教师项目(2000-65)资助 |
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| 摘 要： | 目的 探讨WNK4基因突变与原发性高血压的关系及WNK4基因的表达分析。方法 通过测序在小样本中找到cSNP,然后利用PCR—RFLP方法检测98例原发性高血压患者和95例健康对照者该位点多态。应用RT—PCR方法对WNK4基因进行表达分析。结果 发现了WNK4基因第七外显子G1662A的1个cSNP,且其等位基因频率在原发性高血压组和健康对照组中存在显著性差异。WNK4基因表达分析显示WNK4基因在胎儿肾脏、脑、肺、心、脾、肠等组织中均有表达。结论 WNK4基因与原发性高血压密切相关。
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| 关 键 词： | WNK4基因 原发性高血压 等位基因频率 |
| 修稿时间： | 2002-10-23 |
Correlation analysis between WNK4 gene and essential hypertension |
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| Sun Zhi-jun,Wang Xin-nan,Lu Jing-yu,Ding Qian,Dong Ling-yue,Zhao Yan-yan. Correlation analysis between WNK4 gene and essential hypertension[J]. Acta Academiae Medicinae Sinicae, 2003, 25(2): 145-148 |
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| Authors: | Sun Zhi-jun Wang Xin-nan Lu Jing-yu Ding Qian Dong Ling-yue Zhao Yan-yan |
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| Affiliation: | Department of Genetics, China Medical University, Shenyang 110001, China. |
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| Abstract: | Objective To investigate association of mutation in WNK4 gene with essential hypertension and to analyze the expression of WNK4 gene. Methods cSNP in WNK4 gene in a small samples was detected by sequencing, then PCR-RFLP was performed in 98 patients with essential hypertension and 95 control subjects. The expression profile of WNK4 gene was tested by RT-PCR. Results A cSNP was detected in WNK4 gene exon7 G1662A, and there were significant differences in the distribution of allele frequency of G1662A between essential hypertension group and control group. WNK4 gene were expressed in the tissues of kidney, brain, lung, heart, spleen and intestine of fetus. Conclusion WNK4 gene is well correlated with essential hypertension. |
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| Keywords: | WNK4 gene essential hypertension allele frequency |
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