| 抗-HCV阳性献血者血清HCV RNA水平与ALT活性的关系 |
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| 引用本文： | 钱惠忠,胡越,李林.抗-HCV阳性献血者血清HCV RNA水平与ALT活性的关系[J].临床输血与检验,2004,6(3):171-172. |
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| 作者姓名： | 钱惠忠 胡越 李林 |
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| 作者单位： | 214021,江苏省无锡市红十字中心血站检验科 |
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| 摘 要： | 目的 分析抗-HCV阳性献血者血清HCV RNA含量与ALT活性的关系。方法 采用ELISA法检测抗-HCV，阳性标本用荧光PCR试剂盒测定HCV RNA，分析标本ALT活性与HCV RNA水平的关系。结果 60份抗-HCV阳性标本经PCR检测后有27份标本含有HCV RNA，HCV RNA拷贝量与ALT活性呈正相关，且标本的HCV RNA含量越多，ALT活性也越高(P＜0．01)。结论 献血者中筛查ALT并结合PCR技术，能减少HCV经血传播的危险。
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| 关 键 词： | 聚合酶链反应 抗-HCV 丙型肝炎病毒核酸 丙氨酸氨基转移酶 |
| 文章编号： | 1671-2587(2004)03-0171-02 |
| 修稿时间： | 2003年12月29 |
Investigaion on the relationship between hepatitis C virus RNA and ALT levels in donors with anti-HCV positive |
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| QIAN Huizhong,HU Yue,LI Lin.Wuxi Red Cross Blood Center,Wuxi.Investigaion on the relationship between hepatitis C virus RNA and ALT levels in donors with anti-HCV positive[J].Journal of Clinical Transfusion and Laboratory Medicine,2004,6(3):171-172. |
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| Authors: | QIAN Huizhong HU Yue LI LinWuxi Red Cross Blood Center Wuxi |
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| Institution: | QIAN Huizhong,HU Yue,LI Lin.Wuxi Red Cross Blood Center,Wuxi 214021 |
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| Abstract: | Objective To investigate the relationship betweem serum hepatitis C virus RNA and ALT levels in anti-HCV positive samples of donors. Methods Hepatitis C virus fluorescence PCR diagnostic kit was carried out on the anti-HCV positive samples in order to reveal any possible relationship between HCV RNA and ALT levels. Results 27 samples were tested positive for HCV RNA among 60 anti-HCV positive samples. The more copies of HCV RNA in the blood samples, the higher the ALT levels(P<0.01). Conclusion The combination of ALT testing with HCV RNA PCR in donor screening can reduce the risk of transmission of HCV through blood transfusion. |
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| Keywords: | PCR anti-HCV HCV RNA Alanine aminotransferase |
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