| 临床常见致病菌快速诊断用芯片的初步构建 |
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| 引用本文： | 康素明,潘忠诚,张玉魁,何群,马佳明,赵雨杰. 临床常见致病菌快速诊断用芯片的初步构建[J]. 中国医科大学学报, 2005, 34(4): 299-300,303 |
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| 作者姓名： | 康素明 潘忠诚 张玉魁 何群 马佳明 赵雨杰 |
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| 作者单位： | 1. 中国实用乡村医生杂志编辑部
2. 中国医科大学基础医学院生物芯片中心,辽宁,沈阳,110001 |
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| 摘 要： | 目的:构建针对21种常见致病菌的快速诊断用芯片.方法:针对细菌的高度保守区域设计特异探针,优化条件下与细菌的PCR扩增产物进行杂交.结果:得到特异的杂交结果.结论:芯片方法可用于快速鉴别不同种类的细菌,该方法简便可行,优于传统诊断方法.
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| 关 键 词： | 常见致病菌 诊断芯片 |
| 文章编号： | 0258-4646（2005）04-0299-02 |
| 收稿时间： | 2005-03-01 |
| 修稿时间： | 2005-03-01 |
Construction of diagnostic microarray for 21 kinds of common pathogenic bacteria |
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| KANG Su-ming,PAN Zhong-cheng,ZHANG Yu-kui,HE Qun,MA Jia-ming,ZHAO Yu-Jie. Construction of diagnostic microarray for 21 kinds of common pathogenic bacteria[J]. Journal of China Medical University, 2005, 34(4): 299-300,303 |
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| Authors: | KANG Su-ming PAN Zhong-cheng ZHANG Yu-kui HE Qun MA Jia-ming ZHAO Yu-Jie |
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| Abstract: | Objective: To construct DNA microarray for quick detection and identification of 21 kinds of common pathogenic bacteria. Methods: Special probes were designed based on highly conserved region of bacteria. Under the best hybridization condition, products from polymerase chain reaction of bacteria were hybridized with probes on slides. Results: Special hybridization results were obtained. Conclusion: The DNA microarray can be used in quickly diagnosing different kinds of bacteria. This method is convenient and fast, which is better than conventional diagnostic methods. |
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| Keywords: | common pathogenic bacteria diagnostic microarray |
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