外周血单个核细胞TRAIL mRNA和血清sTRAIL水平与HBV感染肝损伤的相关性

目的:探讨慢性乙型肝炎(CHB)患者外周血单个核细胞(PBMCs)肿瘤坏死因子相关凋亡诱导配体(TRAIL)mRNA及血清可溶性TRAIL(sTRAIL)水平与HBV感染肝损伤的关系.方法:采用TaqMan实时荧光定量RT-PCR(FQ- RT-PCR)检测58例慢性肝炎患者外周血PBMCs中TRAIL mRNA水平,采用夹心酶联免疫吸附法分析其血清sTRAIL水平,同时与血清HBV DNA滴度和肝功能相关指标,进行相关性分析.结果:除轻度慢性乙肝外,其他各型慢性乙型肝炎患者血清sTRAIL水平均显著低于健康对照组(t=2.91,P<0.05),各型慢性乙肝之间无显著性差异,与白蛋白(ALB)呈显著正相关(r =0.426,P<0.05),与TBIL、ALT及HBV DNA滴度无相关性.各型慢性乙肝患者PBMCs中TRAIL mRNA水平显著高于健康对照组(t= 28.31.P<0.001),与肝功能指标及血清HBV DNA滴度无相关性.结论:血清sTRAIL水平降低与肝损伤相关,提示单个核细胞膜结合型TRAIL增高与肝损害及乙肝病程迁延不愈有关.

Involvement of tumor necrosis factor-related apoptosis-inducing ligand mRNA in peripheral blood mononuclear cells and serum soluble tumor necrosis factor-related apoptosis-inducing ligand in liver injury caused by chronic hepatitis B

AIM:To explore the correlations of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) mRNA in peripheral blood mononuclear cells (PBMCs) and serum soluble TRAIL (sTRAIL) in liver injury during chronic hepatitis B (CHB). METHODS:Real-time fluorescence quantitative polymerase chain reaction (FQ-RT-PCR) and sandwich enzyme-linked immunoadsorbent as- say were used to detect the expression of TRAIL mRNA in the PBMCs and the level of sTRAIL in serum of 58 CHB patients,respectively.Then correlation analysis was performed between the results and serum HBV DNA titer,albumin,ala- nine aminotransferase (ALT) and total bilirubin (TBIL) levels. RESULTS:The serum level of sTRAIL in pa- tients with CHB (except for the light type) was significantly lower than that in the healthy controls (t=2.91,P<0.05),but there were no significantly differences among patients with various types of CHB.The level of sTRAIL was positively correlated with serum albumin (r= 0.426,P<0.05),but it had no relationship with HBV DNA titer,TBIL and ALT.The expres- sion of TRAIL mRNA in PBMCs was obviously higher in all the CHB patients than that in the healthy controls (t=28.31,P<0.001),and it had no marked relationship with liver function and HBV DNA titer. CONCLUSION:Lower level of serum sTRAIL may be correlated with CHB-caused liver injury, which indicates that up-regulation of TRAIL expression on the membranes of PBMCs is in- volvement in the delayed course of hepatitis B.