HLA-DRB1*1501和DQB1*0602与新疆哈萨克族HPV 感染及食管癌发生的相关性研究*

目的:从基因水平探讨新疆哈萨克族食管鳞癌的HPV 16E6 感染及食管鳞癌发生与HLA-DRB1，DQB 1 等位基因的遗传易感性，为寻找哈萨克族食管鳞癌的易感基因提供参考。方法:采用聚合酶链反应（PCR ）技术检测200 例哈萨克族食管鳞癌和150 例哈族萨克正常人群HPV 16E6 基因的表达情况，运用序列特异性引物聚合酶链反应技术（PCR-SSP ），检测新疆哈萨克族食管鳞癌患者200 例，哈萨克族正常人群食管膜膜150 例的HLA-DRB1*1501和HLA-DQB1*0602的分布。结果:新疆哈萨克族食管鳞癌患者HPV 16E6 感染率为41% ，明显高于哈萨克族正常人群感染率的14%（P<0.001，OR= 3.94）；HPV 16E6 感染与哈萨克族正常人群HLA-DRB1*1501，HLA-DQB1*0602的无相关性（P>0.05）；新疆哈萨克族食管鳞癌患者HLA-DRB1*1501和HLA-DQB1*0602基因分布频率显著高于哈萨克族正常人群（0.455:0.232，P<0.001，OR= 2.78；0.69比0.554，P=0.006，OR= 1.80）；HLA-DQB1*0602基因阳性率在中低分化鳞癌组中（68.8%）的分布高于高分化鳞癌组（31.2%），差异有统计学意义（P<0.05）。 结论:HPV 16E6 的感染可能是新疆哈萨克族食管癌发生的重要因素之一。HLA-DRB1*1501和HLA-DQB1*0602是哈萨克族食管鳞癌的易感基因，其中HLA-DQB1*0602与哈萨克族食管鳞癌的分化程度有关。 

Study of the Relationship Between HLA DRB1*1501,DQB1*0602 and Xinjiang Kazakh National Minority HPV Infection and Esophageal Carcinoma

Objective: To explore the association between HPV16E6 infection, the polymorphisms of HLA-DRB 1, DQB1 alleles and Kazakh esophageal squamous cell carcinomas in Xinjiang to further provide clues for Kazakh esopha-geal squamous cell carcinoma susceptibility genes. Methods:The HPV16E6 gene infection was detected by polymerase chain reaction (PCR) in 200 Kazakh patients with esophageal squamous cell carcinoma and150 healthy controls . The polymorphisms of HLA-DRB 1DRB1*1501 and HLA-DQB 1*0602 were typed by sequence specific primer by using poly-merase chain reaction (PCR-SSP)in200 Kazakh patients with esophageal squamous cell carcinoma and150 healthy con-trols. Results: Of the patients of Kazakhstan with esophageal squamous cell carcinoma, HPV 16E6 infection rate was 41%, significantly higher than that of ethnic Kazakh population whose infection rate was within the normal range of 14% (P< 0.001 , OR= 3.94); no correlation between the normal controls with Kazakhstan HPV 16E6 infection rate and HLA-DRB 1*1501, DQB 1*0602 was found (P>0.05). Allele frequencies of HLA-DRB 1*1501 and HLA-DQB1*0602 of the patients from Kazakhstan with esophageal squamous cell carcinoma was higher than those in healthy controls (0.455 vs. 0.232 , P<0.001 , OR= 2.78; 0.69vs. 0.554 , P=0.006 , OR= 1.80, respectively). HLA DQB1*0602gene-positive allele in poorly differenti -ated squamous cell carcinoma group ( 68.8 percent) was higher than that of well-differentiated squamous cell carcinoma group (31.2% ), the difference was statistically significant (P<0.05). Conclusion:HPV16E6 infection may be an important factor causing squamous cell carcinoma in the Xinjiang Kazakhstan population. Individuals carrying HLA-DRB 1*1501 and/or DQB1*0602may be more susceptible to esophageal squamous cell carcinoma. HLA-DQB1*0602is related to the differentiation of esophageal squamous cell carcinoma in the Kazakhstani participants.