Evaluation of three real-time PCR assays for detection of Mycoplasma pneumoniae in an outbreak investigation |
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| Authors: | Winchell Jonas M Thurman Kathleen A Mitchell Stephanie L Thacker W Lanier Fields Barry S |
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| Affiliation: | Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. jwinchell@cdc.gov |
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| Abstract: | We compared the performances of three recently optimized real-time PCR assays derived from distinct genomic regions of Mycoplasma pneumoniae during an outbreak. Comprehensive evaluation established that a newly described toxin gene represents a superior target for detecting M. pneumoniae DNA in clinical specimens, although use of multiple targets may increase testing confidence. |
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