龟龄集胶囊对900 MHz手机频率辐射SD雄性大鼠生育力、肝功能和血清乳酸脱氢酶的影响

目的观察龟龄集胶囊对900 MHz 4 h手机频率辐射雄性SD大鼠生育能力、血清乳酸脱氢酶（LDH）和肝功能的影响。方法选育龄期SD雄鼠18只，育龄期SD雌鼠36只。分别将雌、雄性大鼠按体重均衡的原则随机分为3组，即正常组、辐射组、龟龄集组，每组雄鼠6只，雌鼠12只。正常组雄鼠和所有雌鼠不接受辐射，辐射组和龟龄集组雄鼠分别接受4 h/天辐射，连续18天，龟龄集组辐射的同时给予龟龄集胶囊混悬液［0.15 g/（kg·d）］灌胃，其余雄鼠灌等量生理盐水。于辐射之第14~18夜，各组雄雌大鼠按1∶2合笼连续5夜，雄鼠于辐射结束次晨处死，雌鼠正常喂养至分娩前处死。观察比较与雄鼠交配雌鼠妊娠结局（包括妊娠率、死胎数、仔鼠数、出生体重、身长、尾长），雄鼠血清LDH、ALT、AST及AST/ALT， HE染色观察肝脏组织形态学变化。结果与正常组比较，辐射组雌鼠妊娠率降低，死胎数增加；血清LDH明显升高，差异有统计学意义（P〈0.05）；血清ALT、AST水平和AST/ALT无明显变化；肝细胞核缩小以及肝细胞浆空泡样变性。与辐射组比较，龟龄集组雄鼠致雌鼠妊娠率增加，死胎率降低；血清LDH明显下降（P〈0.05）；血清ALT、AST水平和AST/ALT无明显变化；肝细胞核萎缩和细胞浆空泡样变性明显减轻，肝组织形态基本恢复正常。结论900 MHz 4 h手机频率辐射可降低与SD雄性大鼠交配后雌鼠的妊娠率，增加死胎数、使肝脏组织形态学异常，血清LDH水平升高，而龟龄集胶囊可防治手机辐射造成的妊娠率下降和死胎数增加及肝组织形态学损伤，降低血清LDH水平，血清ALT、AST变化无统计学意义。

Effect of GuilingJi Capsule on the Fertility,Liver Functions,and Serum LDH of Male SD Rats Exposed by 900 MHz Cell Phone

Objective To observe the effect of Guilingji Capsule （GC） on the fertility, liver func- tions, and serum lactate dehydrogenase （LDH） of adult male SD rats exposed by 900 MHz cell phone. Methods Totally 18 adult male SD rats and 36 adult female rats in child-bearing period were selected and randomly divided into three groups according to weight equilibrium principle, i.e., the normal group, the ra- diated group, and the GC group, 6 males and 12 females in each group. Male rats in the normal group and all female rats were not radiated. Male rats in the radiated group and the GC group received radiation for 4 h per day, lasting for 18 successive days. Rats in the GC group received GC suspension at the daily dose of 0. 15 g/kg by gastrogavage at the same time. Equal volume of normal saline was administrated to other male rats. Then male rats were mated with corresponding female rats from the 14th radiation night to the 18th radiation night in the ratio of 1：2. Male rats were killed following on the next morning of ending the radiation. Female rats were normally fed and then killed before delivery. The pregnant outcomes of female rats in responding groups （the rates of pregnancy and the number of death fetus, birth weight, body length, and tail length） were observed and compared. Serum alanine aminotransferase （ALT）, aspartate transferase （AST）, AST/ALT, and LDH levels of the male rats were detected by colorimetry. Histological and morpho- logical changes of liver were observed by HE staining. Results Compared with the normal group, the pregnancy rates of female rats decreased and the number of death fetus increased, the serum LDH level obviously increased in the radiated group （P 〈0.05）. Serum levels of ALT, AST, and AST/ALT were no significantly changed in the radiated group. The hepatocyte nuclear atrophy and cytoplasm vacuolar degenera- tion appeared. Compared with the radiated group, the pregnancy rates increased, the number of death fe- tus dropped, and the serum level of LDH decreased in the GC group （P 〈0.05）. There was no obvious change in serum levels of ALT, AST, or AST/ALT. The hepatocyte nuclear atrophy and cytoplasm vacuolar degeneration were significantly attenuated. The histomorphological structures recovered to normal basically in the GC group. Conclusions The pregnancy rates could be decreased, the number of death fetus increased, histomorphological structures abnormal, and serum LDH level increased by exposure toy GSM 900 MHz cell phone. GC could prevent and treat the aforesaid lesion. But there was no statistical difference in serum ALT or AST levels.