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| 尾悬吊大鼠骨和骨髓中骨钙素的变化 | |
| 引用本文： | 付崇建,郁冰冰,杨连甲,张立藩.尾悬吊大鼠骨和骨髓中骨钙素的变化[J].航天医学与医学工程,2003,16(4):260-263. |
| 作者姓名： | 付崇建 郁冰冰 杨连甲 张立藩 |
| 作者单位： | 1. 济南军区总医院口腔科,山东济南,250031 2. 第四军医大学口腔医学院病理科,陕西西安,710032 3. 第四军医大学航空航天医学系,陕西西安,710032 |
| 基金项目： | 全军医药卫生科研基金资助项目 (98Z0 83 ) |
| 摘 要： | 目的研究模拟失重骨质和骨髓内骨钙素 (OC)以及骨和软骨钙盐代谢的形态学变化。方法选用2 0只SD大鼠 ,随机配对分为 1 4d和 2 8d尾悬吊组及相应的 2个自由活动组 ,每组 5只。组织标本行原位杂交及三色染色。结果尾吊组大鼠骨质和骨髓内OC的表达明显弱于对照组 (P <0 .0 5 ) ;尾吊组1 4dOC的表达明显强于 2 8d(P <0 .0 5 )。模拟失重导致股骨和软骨基质钙化障碍 ,原有钙化基质内钙盐脱钙明显 ,2 8d脱钙明显加重。结论大鼠后肢去负荷导致骨和骨髓OC含量的降低 ,骨和软骨钙盐代谢障碍 ,去负荷时间越长越显著 |
| 关 键 词： | 失重模拟 尾吊 骨钙素 骨 骨髓 原位杂交 |
| 文章编号： | 1002-0837(2003)04-0260-04 |
| 修稿时间： | 2002年8月17日 |
Changes of Osteocalcin in Bone and Bone Marrow in Tail Suspended Rats |
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| FU Chong jian,YU Bing bing,YANG Lian jia,ZHANG Li fan.Changes of Osteocalcin in Bone and Bone Marrow in Tail Suspended Rats[J].Space Medicine & Medical Engineering,2003,16(4):260-263. | |
| Authors: | FU Chong jian YU Bing bing YANG Lian jia ZHANG Li fan |
| Institution: | Department of Stomatology, Jinan Military General Hospital, Jinan, China. |
| Abstract: | Objective To study osteocakin(OC) changes in bone and marrow and calcium deposition in bone and cartilage under simulated weightlessness. Method Twenty SD rats were randomly divided into 14 d and 28 d tail suspension group and 2 corresponding control groups. Histological samples were in situ hybridized and trichrome stained. Result OC expression of bone and marrow of rats were lower in tail suspended rats than that in the control(P<0.05). OC expression in 14 d tail suspended rats were higher than that in 28 d tail suspended group(P<0.05). Mineralization was inhibited, and demineralization of furmer and cartilage mineralized matrix was prominent. Demineralization was more prominent in 28 d group.Conclusion OC levels in bone and marrow of rats were lower after tail suspension. Calcium deposition was inhibited in bone and cartilage. Demineralization was prominent after long term hindlimb unloading. |
| Keywords: | weightlessness simulation tail suspension osteocalcin bone bone marrow in situ hybridization |
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