胃癌细胞中RHAMM基因表达的对比分析

目的 分析胃癌发生中透明质酸（ｈｙａｌｕｒｏｎｉｃ ａｃｉｄ，ＨＡ）介导的细胞游走受体（ｒｅｃｅｐｔｏｒ ｏｆ ＨＡ ｍｅｄｉａｔｅｄ ｍｏｔｉｌｉｔｙ，ＲＨＡＭＭ）的表达形式以及ＨＡ对其表达的影响。方法 采用免疫组织化学和Ｗｅｓｔｅｒｎ印迹杂交方法，分析ＲＨＡＭＭ蛋白在１０例萎缩性胃炎、１２例肠上皮化生、２０例Ⅲ度非典型增生、７４例各类型胃癌／２析胃癌细胞系以及３６例非癌粘膜临床标本中的表达形式

Alternative splicing of RHAMM gene in Chinese gastric cancers and its in vitro regulation

Objective To identify the receptor of hyaluronic acid mediated motility (RHAMM), expression pattern(s) in the gastric mucosa with various lesions and to elucidate the effect of hyaluronic acid(HA) on in vitro RHAMM expression. Methods Immunocytochemical staining and Western blot hybridization were performed to check the expression patterns and locations of RHAMM molecules in the noncancerous, preneoplastic and malignant gastric specimens. RHAMM expression of the two stomach cancer cell lines cultured conventionally or under the conditions with gelatin or HA supplementation was evaluated as well. Results Mr 93 000 95 000 intracellular RHAMM, so called IHABP, proteins were detected in 17% noncancerous mucosa. The multiple spectrums and increased frequencies of RHAMM expression could be observed in the majority of the cancer cases with both intracellular and cell surface labeling. HA but not gelatin could promote membrane type RHAMM (mRHAMM) expression of stomach cancer cells in a dose associated pattern. Conclusion The expression of mRHAMM variants is closely associated with tumor progression, which may be promoted and modulated by HA.