| 紫草抗人乳头瘤病毒作用的研究 |
| |
| 引用本文： | 符惠燕,邓远辉,冯怡,孙静,周丹,王海兰.紫草抗人乳头瘤病毒作用的研究[J].中药新药与临床药理,2005,16(4):259-260. |
| |
| 作者姓名： | 符惠燕 邓远辉 冯怡 孙静 周丹 王海兰 |
| |
| 作者单位： | 1. 广州中医药大学,广州,510407
2. 广州中医药大学第二附属医院,广州,510120 |
| |
| 基金项目： | 广东省医药卫生科研项目 |
| |
| 摘 要： | 目的筛选紫草抗人乳头瘤病毒(HPV)的有效部位.方法用系统溶媒(石油醚、正丁醇、乙醇、蒸馏水)对紫草依次进行提取,应用荧光定量PCR技术检测在体外的抗HPV-DNA的效果.结果仅紫草水提物显示在体外抑制HPV-DNA活性,最低有效浓度为0.08 g/mL.结论紫草具有体外抑制HPV-DNA作用,其水提物中存在抗HPV-DNA活性成分.
|
| 关 键 词： | 紫草提取物 人乳头瘤病毒 荧光定量聚合酶链反应 |
| 文章编号： | 1003-9783(2005)04-0259-02 |
| 修稿时间： | 2005年1月20日 |
Studies of Anti - Human Papillomavirus Activity of Herba Arnebia |
| |
| FU Huiyan,DENG Yuanhui,FENG Yi,SUN Jing,ZHOU Dan,Wang Hailan.Studies of Anti - Human Papillomavirus Activity of Herba Arnebia[J].Traditional Chinese Drug Research & Clinical Pharmacology,2005,16(4):259-260. |
| |
| Authors: | FU Huiyan DENG Yuanhui FENG Yi SUN Jing ZHOU Dan Wang Hailan |
| |
| Abstract: | Objective To select the herba anti- human papillomavirus (HPV) active fraction from herba Arnebia. Methods The fractions from herba Arnebia. were separated with systematic solvents including petroleum arieal part of benzin, n- butanol , ethanol and distilled water, and their effects on HPV- DNA were evaluated by fluorescence quantitative polymerase chain reaction (FQ- PCR) technique. Results Only the water- extract of.this drug showed in- vitro inhibitory effect on HPV- DNA and its minimum effective concentration is 0.08g/mL. Conclusion Herba Arnebia. has in- vitro inhibitory effect on HPV- DNA and the active components exists in the water- extract. |
| |
| Keywords: | Herba Arnebia Human papillomavirus Fluorescence quantitative polymerase chain reaction |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中药新药与临床药理》浏览原始摘要信息 |
| 点击此处可从《中药新药与临床药理》下载免费的PDF全文 |
