| 卵巢癌顺铂耐药细胞模型中14-3-3蛋白的差异表达 |
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| 引用本文： | 王慧香,翟建军,王蕾,侯丽娟,孙燕.卵巢癌顺铂耐药细胞模型中14-3-3蛋白的差异表达[J].中国实用妇科与产科杂志,2011(7). |
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| 作者姓名： | 王慧香 翟建军 王蕾 侯丽娟 孙燕 |
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| 作者单位： | 首都医科大学附属北京同仁医院妇产科; |
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| 摘 要： | 目的探讨卵巢癌顺铂化疗耐药模型中14-3-3蛋白的差异表达,为深入研究卵巢癌顺铂耐药机制提供理论依据。方法分别提取卵巢癌顺铂耐药和敏感细胞的蛋白质,应用差异凝胶电泳(DIGE),通过DecyderTM分析软件获得差异表达蛋白质点,进行基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF-MS)分析、鉴定。结果经过二维电泳技术分离,MALDI-TOF-MS成功鉴定出2个14-3-3蛋白亚型,即14-3-3σ和14-3-3ζ,在耐药细胞中表达分别上调31%和38%,t检验P值分别为0.0049和0.0032。结论差异蛋白14-3-3σ和14-3-3ζ的鉴定为探讨这类蛋白在卵巢癌顺铂耐药中的作用奠定了基础,并提供了候选治疗靶点。
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| 关 键 词： | 卵巢癌 耐药 14-3-3蛋白 |
Differential expression of 14-3-3 proteins in platinum resistance ovarian cancer cell model |
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| WANG Hui-xiang,ZHAI jian-jun,WANG Lei,HOU Li-juan,SUN Yan.Differential expression of 14-3-3 proteins in platinum resistance ovarian cancer cell model[J].Chinese Journal of Practical Gynecology and Obstetrics,2011(7). |
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| Authors: | WANG Hui-xiang ZHAI jian-jun WANG Lei HOU Li-juan SUN Yan |
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| Institution: | WANG Hui-xiang,ZHAI jian-jun,WANG Lei,HOU Li-juan,SUN Yan. Department of Obstetrics and Gynecology,Beijing Tongren Hospital,Capital University of Medical Science,Beijing 100176,China |
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| Abstract: | Objective To investigate the differential expression of 14-3-3 proteins in platinum resistance and platinum sensitive ovarian cancer cell clones.Methods The proteins of C0C1 and C0C1/DDP were separated by highly sensitive two-dimensional differential gel electrophoresis(2-D DIGE) coupled with Matrix-assisted laser desorption/ionization-time of flight(MALDI-TOF-MS) for the identification of protein differentially expressed in cisplatin sensitive and cisplatin resistance cell lines.Results We found a group of... |
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| Keywords: | ovarian cancer drug resistance 14-3-3 proteins |
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