葛根散对急性酒精性肝损伤小鼠肝微粒体 CYP450含量及CYP2E1活性的影响

目的:考察经典解酒方葛根散对急性酒精性肝损伤小鼠肝微粒体细胞色素P450( CYP450)含量及细胞色素P450-2E1( CYP2E1)活性的影响.方法:实验小鼠分葛根散高、中、低剂量组、正常对照组和模型对照组,各给药组分别给予葛根散水煎剂(按生药量计,20,10,5 g·kg-1)ig,对照组代以等体积蒸馏水,每天1次,连续10 d；每天给药30 min后,模型对照组和各给药组以15 mL·kg-1 56％白酒ig,正常对照组代以等体积蒸馏水,以血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)及肝脏系数为参考指标,制备急性酒精性肝损伤小鼠模型；取小鼠肝组织检测葛根散对肝微粒体CYP450含量及CYP2E1活性的影响.结果:与正常对照组比较:模型对照组血清ALT、AST水平及肝脏系数显著升高(P＜0.01),肝微粒体蛋白含量无明显变化,CYP450含量及CYP2E1活性显著提高(P＜0.01).与模型对照组比较:葛根散各剂量组血清ALT,AST水平及大剂量组肝脏系数呈明显降低(P ＜0.05或P＜0.01),肝微粒体蛋白含量无明显变化,CYP450含量明显增多(P＜0.01)且有剂量差异性(P＜0.05),CYP2E1活性显著降低(P＜0.01)且有剂量差异性(P＜0.01).结论:葛根散对急性酒精性肝损伤小鼠肝功能具有良性干预作用,能提高酒精性肝损伤小鼠肝微粒体CYP450含量和降低CYP2E1活性.

Effect of Gegen Powder on CYP450 Content of Liver Microsomal and Activity of CYP2E1 in Mice Acute Alcohol-induced Liver Injury

Objective: To observe the effects of Jiejiu recipes of Gegen powder on the cytochrome P450(CYP450) content of liver microsomal and the activity CYP2E1 in mice acute alcohol-induced liver injury model. Method: Mouse were divided into normal control group, model group and Gegen powder high, medium nad low dose groups. Gegen powder of 20,10, 5 g·kg^-1,was given once a day and continuing ten days.After 30 minutes of administration everyday, the mouse of model group and treatment groups were orally given 56% alcohol of 15 mL·kg^-1 to establish acute alcohol-induced liver injury model, and the level of ALT, AST in serum and liver index were observed; Content of CYP450 and the activity of CYP2E1 were detected. Result: Compared with normal control group, the level of ALT, AST and liver index in model group had obvious enhance(P<0.01), the content of microsomal protein had no significant change, the content of CYP450 and the activity of CYP2E1 were significantly raised(P<0.01). Compared with model group, the level of ALT, AST and liver index in Gegen powder groups had obvious reduction(P<0.05 or P<0.01), the content of microsomal protein had no significant change, the content of CYP450 was increased significantly (P<0.01),the activity of CYP2E1 was significantly decreased (P<0.01) and there was a significant difference between different dosage groups (P<0.05). Conclusion: Gegen powder indicates the therapeutic effect on acute alcohol-induced liver injury, it can be contribute to increaseing the content of CYP450 and reduceing the activity of CYP2E1