| 盆腔器官脱垂患者骶韧带成纤维细胞粘弹性研究 |
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| 引用本文： | 单淑芝,石彬,吴燕菁,李晓娟,王亚冰. 盆腔器官脱垂患者骶韧带成纤维细胞粘弹性研究[J]. 中国实用妇科与产科杂志, 2011, 0(7) |
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| 作者姓名： | 单淑芝 石彬 吴燕菁 李晓娟 王亚冰 |
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| 作者单位： | 河北医科大学第二医院妇产科; |
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| 基金项目： | 河北省科技支撑计划项目(092061107D) |
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| 摘 要： | 目的探讨盆腔器官脱垂(pelvic organ prolapse,POP)患者骶韧带成纤维细胞粘弹性变化。方法选取2009年12月至2010年6月河北医科大学第二医院妇产科POP患者11例为观察组,同期非POP患者13例为对照组,取骶韧带进行成纤维细胞的原代培养。光镜观察与免疫细胞化学染色法进行成纤维细胞鉴定,取1代的成纤维细胞(实验组观察细胞数为18,对照组观察细胞数为22),采用微管吸吮技术表征骶韧带成纤维细胞的粘弹性特性。结果培养的细胞呈梭形,表达波形蛋白(vimentin),POP患者骶韧带成纤维细胞粘弹特性明显低于正常对照组,病例组骶韧带成纤维细胞平衡模量[E∞=(124.62±20.22)Pa]、瞬间模量[E0=(429.25±105.89)Pa]、黏性系数[μ=(718.53±159.08)Pa.s]均显著低于对照组[E∞=(170.17±24.65)Pa]、[E0=(495.26±69.39)Pa、μ=(845.35±155.34)Pa.s],P<0.05。结论 POP患者成纤维细胞骨架结构和机能状态的改变可能是POP发生的关键环节。
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| 关 键 词： | 盆腔器官脱垂 微管吸吮 成纤维细胞 粘弹特性 |
Viscoelastic properties of fibroblasts of human uterosacral ligament in pelvic floor dysfunction |
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| SHAN Shu-zhi,SHI Bin,WU Yan-jing,LI Xiao-juan,WANG Ya-bing. Viscoelastic properties of fibroblasts of human uterosacral ligament in pelvic floor dysfunction[J]. Chinese Journal of Practical Gynecology and Obstetrics, 2011, 0(7) |
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| Authors: | SHAN Shu-zhi SHI Bin WU Yan-jing LI Xiao-juan WANG Ya-bing |
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| Affiliation: | SHAN Shu-zhi,SHI Bin,WU Yan-jing,LI Xiao-juan,WANG Ya-bing. Department of Obstetrics and Gynecology,the Second Hospital of HeBei Medical University,Shijiazhuang 050000,China |
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| Abstract: | Objective To characterize the changes of the viscoelasticity of fibroblasts derived from uterosacral ligament of pelvic organ prolapse and nomal ones.Methods 11 patients with POP and 13 patients without POP who underwent hysterectomy surgery in the Obstetric and Gynecological Department of the Second Hospital of Hebei Medical University between December 2009 and June 2010 were recruited in this study,fibroblasts of experimental group(n=18) and control group(n=22) were observed respectively.Uterosacral ligam... |
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| Keywords: | pelvic organ prolapse micropipette aspiration fibroblast viscoelasticity |
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