恒山黄芪内生真菌Aspergillus sp.代谢产物的分离和生物活性的测定

目的:研究恒山黄芪内生真菌的代谢产物.方法:采用反复硅胶柱色谱法、Sephadex LH-20凝胶色谱法等进行分离纯化,并通过理化常数测定和光谱分析鉴定其化学结构.结果:从恒山黄芪一株内生真菌Aspergillus sp.的发酵液中分离得到6个代谢产物,经波谱解析,分别为cerevisterol(1),ergosterol peroxide(2),fumitremorgin B(3),verruculogen(4),fumitremorgin C(5)和cyclotryprostatins B(6).抗菌活性显示,6个化合物对大部分测试菌均具有明显的抑制活性,特别是化合物6显示出很强的抑制真菌的活性.结论:所有化合物均首次从恒山黄芪内生真菌中分离得到.

Isolation and Biological Evaluation of Secondary Metabolites of Endophytic Fungus Aspergillus sp. from Astragalus membranaceus

Objective: To study on secondary metabolites from an endophytic fungus of Astragalus membranaceus. Method: The compounds were isolated by column chromatography and identified on the basis of physic-chemical constants and spectral analysis. Result: Six compounds were obtained and elucidated as cerevisterol (1), ergosterol peroxide (2), fumitremorgin B (3), verruculogen (4), fumitremorgin C (5) and cyclotryprostatins B (6). Biological evaluation showed that the compounds displayed significantly inhibitory activities. Especially, compound 6 had strong antifungal activity. Conclusion: The six compounds were obtained from an endophytic fungus Aspergillus sp. of A. membranaceus for the first time.