龙柴方中黄芩、垂盆草总黄酮的提取纯化工艺

目的:优选龙柴方中黄芩、垂盆草总黄酮的提取纯化工艺.方法:以总黄酮含量为指标,采用正交试验法优选总黄酮提取纯化工艺条件.大孔树脂纯化总黄酮,以静态吸附及解析试验筛选树脂型号;通过动态吸附相关指标考察最佳洗脱条件.结果:最佳提取工艺为15倍量60％乙醇,85℃水浴回流提取2次,每次2h.最佳纯化工艺条件为采用AB-8型大孔树脂,上样浓度为0.11 g·mL-1,吸附速度3 BV·h-1,5 BV水洗除杂,8 BV 70％乙醇洗脱.结论:优选工艺得到的总黄酮含量较高,工艺简单,适宜工业化生产.

Extraction and Purification Technology of Total Flavonoids for Scutellaria baicalensis and Sedum sarmentosum from Longchaifang

Objective:To optimize extraction and purification technology of total flavonoids for S.baicalensis and S.sarmentosum from Longchaifang.Method:The content of total flavonoids were selected as index,extraction and purification technology condition of total flavonoids was optimized by orthogonal test.Total flavonoids was purified by macroporous resin,screened optimum purification macroporous resin by static adsorption and desorption test;Optimum elution condition was examined by related indicators of dynamic adsorption.Result:Optimum extraction process was as follows:reflux extracted 2 times with 15 times the anount of 60% ethanol at 85 ℃,2 h each time.Optimum purification technology was as follows:AB-8 macroporous resin was adopted,concentration of sample was 0.11 g·mL-1,adsorptive speed was 3 BV·h-1,adsorbing column was eluted with 5 BV waterand 8 BV 70% ethanol.Conclusion:The content of the total flavonoids was high by optimization technology.This technique was simple and suitable for modern production.