HPLC-MS识别苦木中苦参碱和氧化苦参碱

目的:利用高效液相色谱-串联质谱联用分析和鉴定苦木中苦参碱及氧化苦参碱.方法:采用高效液相色谱-电喷雾/四极杆-飞行时间串联质谱,通过与对照品保留时间、准确分子量及其一级、二级质谱比对,进行成分鉴定,并建立了高效液相色谱-串联四级杆质谱联用测定苦木中苦参碱与氧化苦参碱的定量方法研究.结果:苦木中含有微量的苦参碱及氧化苦参碱成分,建立的方法专属、灵敏,可作为苦木中苦参碱及氧化苦参碱的识别依据.结论:首次以LC-MS鉴别出了苦木中苦参碱与氧化苦参碱.不同产地不同部位的苦木药材中苦参碱与氧化苦参碱含量差别较大.

Identification of Matrine and Ammothamnine in Picrasmae Lignum by High Performance Liqid Chromatography/Tandem Mass spectromety

Objective: Identification of matrine and ammothamnine in picrasmae lignum by high performance liqid chromatography /Tandem Mass spectrometry. Method: Two alkaloids in picrasmae lignum were ananlyzed by HPLC-ESI/Q-TOF MS/MS. According to the accurate molecular weight, retention time and MSn data by compared with reference substances, the chemical structures were identified. After that, quantitative method was built based on the techniques of LC-Q-Q-Q. Result: Two trace components were identified in picrasmae lignum. The method is specific, sensitive and can be used as the identification of matrine and oxymatrine in picrasmae lignum. Conclusion: It was the first time to report the identification of Matrine and ammothamnine in picrasmae lignum by LC-MS. The content of matrine and ammothamnine in picrasmae lignum vary greatly with the different part and the area of produce.