均匀设计法优选党参茯苓水溶性多糖的微波提取工艺

目的:优选微波法提取党参、茯苓混合水溶性多糖的适宜条件.方法:采用均匀设计法考察微波功率、提取时间、料液比和提取次数4个因素对混合多糖含量的影响.结果:最佳微波提取工艺条件为微波功率130 W,提取时间6 min,料液比1:40 (g·mL-1),提取3次.微波混合多糖的提取率15.79％；传统混合多糖的提取率为15.11％.红外光谱图分析表明微波提取基本没有破坏多糖的结构.结论:微波提取的混合多糖含量和传统提取的混合多糖含量无显著性差异,但微波提取迅速、方法简便,适合工业化生产.

Optimization of Microwave Extraction Technology for Water Soluble Polysaccharides from Codonopsis pilosula and Poria Cocos by Uniform Design Method

Objective:To optimize microwave extraction conditions of mixed water-soluble from Codonopsis pilosula and Poria cocos.Method:Uniform design method was usd to investigate effects of microwave power,extraction time,ratio of solid-solvent and extraction times on the content of mixed polysaccharide.Result:Optimum microwave extraction technology conditions were as follows:microwave power was 130 W,extraction time was 6 min,ratio of solid-solvent was 1:40(g·mL-1),extraction times was 3 times,yield of mixed water-soluble polysaccharide was 15.79% by microwave extraction,and it was 15.11% by conventional extraction method.Conclusion:Microwave extraction and conventional extraction of mixed polysaccharide showed no significant difference,but because of microwave extraction method was rapid,simple,it was suitable for modern production.