抗炎宫乐胶囊治疗血热型崩漏的机制

目的:探讨抗炎宫乐胶囊治疗血热型崩漏的机制.方法:雌性ICR小鼠按体重随机分为模型组、空白组、抗炎宫乐胶囊高、中、低(1.2,0.6,0.3 g·kg-1)剂量组、宫血宁组、布洛芬组,分别ig给药5,10,10 d.雌性SD大鼠分组情况同小鼠,抗炎宫乐胶囊高、中、低(0.94,0.47,0.24 g·kg-1)剂量组,ig给药20,7,30 d.通过测定抗炎宫乐胶囊对断尾出血时间、血液流变学指标、血小板聚集功能、抗炎镇痛、促性腺激素和性激素调节的影响,研究其治疗血热型崩漏的机制.结果:抗炎宫乐胶囊能够明显缩短小鼠断尾出血时间(P＜0.05)；缩短大鼠血浆凝血酶原时间(PT)和凝血活酶时间(APTT) (P ＜0.05),增强二磷酸腺苷(ADP)诱导大鼠血小板聚集功能(P＜0.05)；减小二甲苯所致小鼠耳肿胀度(P＜0.05)和子宫异物埋植所致大鼠子宫肿胀度(P＜0.05)；延长小鼠疼痛扭体的潜伏期和减少疼痛扭体的次数(P＜0.05)；提高去卵巢大鼠血清中卵泡刺激素(P＜0.05)、黄体生成素(P＜0.05)、雌二醇水平(P＜0.05)和降低血清中孕酮水平(P＜0.05).结论:抗炎宫乐胶囊对模型动物具有明显的止血、抗炎、镇痛和调节下丘脑-垂体-性腺轴的作用,其治疗血热型崩漏的作用及机制可能与上述环节有关.

Mechanism on Treatment of Metrorrhagia due to Blood-Heat by Kangyan Gongle Capsule

Objective:To study the mechanism on treatment of metrorrhagia due to blood-heat by Kangyan Gongle capsule.Method:Female ICR mouse were randomized into model group,control group,Kangyan Gongle capsule groups treated with dose of 1.2,0.6,0.3 g·kg-1,Gongxuening group and Ibuprofen group,treatment lasted 5,10 and 10 days,respectively.Female SD rats were randomized into the groups as the above mentioned.The dose of Kangyan Gongle capsule was 0.94,0.47,0.24 g·kg-1 accordingly.The treatments(ig) lasted 20,7,30 days,respectively.Bleeding time,hemorheology,platelet aggregation rate,regulating gonadotrophin and gonadal hormone,anti-inflammatory and analgesic effect were determined to study the mechanism.Result:Kangyan Gongle capsule could obviously shorten the bleeding time of mice broken tail(P<0.05),shorten prothrombin time(PT) and activated partial thromboplastin time(APTT)(P<0.05),enhance platelet aggregation rate(P<0.05),decrease the degree of mice ear edema caused by xylene(P<0.05) and rat uterus swelling by embedded foreign matter(P<0.05),relieve mice’ pain caused by oxytocin(P<0.05),upregulate the level of follide stimulating hormone(FSH)(P<0.05),luteinizing hormone(LH)(P<0.05),estradiol(E2)(P<0.05) and downregulate the level of P(P<0.05) in ovariectomized rat’ serum.Conclusion:Kangyan Gongle capsule obviously indicates hemostatic,antiinflammatory,analgesic effect and could regulate the function of hypothalamus-pituitary-gonad axis,which.May be included into the mechanism of actions.