壮肾1号颗粒治疗阿霉素肾病大鼠蛋白尿的作用

目的:观察壮肾1号颗粒对阿霉素肾病大鼠蛋白尿的影响,并从生化、分子生物学角度探讨其作用机制.方法:SD雄性大鼠用尾静脉1次性注射阿霉素(0.006 g·kg-1)制备肾炎蛋白尿模型.大鼠分为5组,分别为空白对照组、模型对照组、洛汀新组(0.003 g·kg-1)、壮肾1号颗粒低、高剂量组(2.52,10.08 g·kg-1),造模7d后开始ig给药,每日1次,疗程8周,检测大鼠24 h尿蛋白定量、β2-微球蛋白(β2-MG),取血放射免疫法检测肿瘤坏死因子-α(TNF-α).结果:实验8周末各治疗组(壮肾1号颗粒高、低剂量组及洛汀新组)阿霉素肾病大鼠24 h尿蛋白定量下降,以壮肾1号颗粒高剂量组最为明显(P＜0.05)；尿β2-MG的含量降低,以壮肾1号颗粒高剂量组最为明显(P＜0.05)；阿霉素肾病大鼠TNF-α含量降低,以壮肾1号颗粒高剂量组最为明显(P＜0.05).结论:壮肾1号颗粒对肾小球、肾小管有保护作用；通过影响细胞因子调节网络、减轻炎症反应减少尿蛋白为其可能机制；高剂量组较中药低剂量组、洛汀新组疗效好.

Modulation of Zhuangshen Yihao Granule on Proteinuria Induced by Adriamycin in Rats

Objective:To observe the effects of Zhuangshen Yihao granule on proteinuria excreting in nephrosis rats induced by adriamycin(ADR),and discuss the mechanism in biochemistry and molecular biology.Method:The proteinuria model of nephrosis rats was established by injecting ADR(0.006 g·kg-1) by the tail vein.And then they were randomly divided into groups as follows:model group,lotensin group(0.003 g·kg-1),low-dose group(2.52 g·kg-1) and high-dose group(10.08 g·kg-1) of Zhuangshen Yihao granule,and normal group was set up meanwhile,drugs were given accordingly once a day after 7 days of modeling,administration lasted 8 weeks.At the 8th week of he experiment,24 hours urinary protein was assayed,urine β2-MG and tumor necrosis factor(TNF)-αin blood serum were detected by radioimmunoassay.Result:Levels of 24 hours urinary protein,urine β2-MG and the content of TNF-α of serum in lotensin group,the low-dose and the high-dose groups of Zhuangshen Yihao granule were reduced,especially in the high-dose group of Zhuangshen Yihao granule(P<0.05).Conclusion:Zhuangshen Yihao granule can exerted a protective effect on glomerulus and renal tubule,and revealed that producing an effect on the regulation network of cytokines,and abating inflammatory reaction may be its possible mechanism.Furthermore,the above curative effects of Zhuangshen Yihao granule in the high-dose group were better than in the low-dose group and the lotensin group.