加味逍遥汤对抑郁大鼠海马cAMP,PKA,PKC的影响

目的:从信号转导通路上重要调节因子cAMP(环磷酸腺苷),PKA(蛋白激酶A)及PKC(蛋白激酶C)的角度,探讨抑郁大鼠模型受体后信号转导的变化及加味逍遥汤的干预作用.方法:将SD大鼠随机分为6个组,每组10只,即正常组、模型组、氯米帕明组(13.5 mg·kg-1)、加味逍遥汤高、中、低剂量组(生药11.7,5.85,2.925 g·kg-1).造模同时各组按10 mL·kg-1体重给药,正常组、模型组给予等量的生理盐水,每日1次,连续24 d.末次给药后1h,快速断头处死,在冰上剥离大脑,分离海马迅速放入冻存管,投入液氮中,随后置于-70℃低温冰箱保存备用.按ELISA试剂盒说明用酶标仪检测大鼠海马cAMP,PKA及PKC的含量变化.结果:与模型组比较,各组均能增加大鼠海马cAMP,PKA及PKC的含量,差异有显著性意义(P＜0.05),加味逍遥汤各剂量组cAMP,PKA及PKC的含量增加,随剂量增加作用加强,与模型组比较差异有显著性意义(P＜0.05)；加味逍遥汤高、中剂量组与氯米帕明组相比cAMP,PKA及PKC含量差异没有显著性意义.加味逍遥汤高、中剂量组与低剂量组相比,差异有显著性意义(P＜0.05).结论:加味逍遥汤可能是通过调节受体后cAMP,PKA及PKC信号传导通路发挥抗抑郁作用.

Effect of Modified Jiawei Xiaoyao Tang on cAMP,PKA and PKC in Rat Hippocampus with Depression

Objective:To explore The effect of modified Jiawei Xiaoyao Tang on cyclic adenosine monophosphate(cAMP),protein kinase A(PKA) and protein kinase C(PKC) in rat hippocampus with depression.Method:Rats were randomly divided into six groups:control group,model group,clomipramine group(13.5 mg·kg-1),modified Jiawei Xiaoyao Tang of high,medium and low dose group(11.7,5.85,2.925 g·kg-1).Each group was administrated continuously for 24 ds.After 1 hour of administration,the hippocampus was separated rapidly into the freezing tube and placed in-70 ℃ refrigerator to detecte the level of cAMP,PKA and PKC by ELISA.Result:Compared with model group,each group of modified Jiawei Xiaoyao Tang significantly increased level of cAMP,PKA and PKC(P<0.05) with dose-depandent manner.Compared with clomipramine group,high and medium dose showed the no significant differences.Conclusion:The anti-depression effect of modified Jiawei Xiaoyao Tang may be related to regulating the signal transduction of cAMP,PKA and PKC.