丹参水提物对心肌缺血/再灌注损伤大鼠心率及心肌酶谱的影响

目的:观察丹参水提物对心肌缺血/再灌注(MI/R)损伤大鼠心率及心肌酶谱的影响.方法:SD大鼠随机分为6组:假手术组、模型组、丹参注射液(含生药4 g·kg-1)组、丹参水提物高、中、低剂量(12.8,6.4,3.2 mg·kg-1)组,每组8只.采用在体大鼠结扎冠状动脉前降支缺血30 min后,松扎再灌注30 min,造成MI/R损伤模型,于缺血最后5 min持续到再灌注前5 min通过股静脉推注给予,假手术组模型组予生理盐水.观察丹参水提物对MI/R损伤大鼠模型心率及心肌酶谱的影响.结果:丹参水提物可显著抑制MI/R引起的大鼠心率减慢,显著减少MI/R损伤大鼠血清中天门冬氨酸氨基转移酶(AST)、乳酸脱氢酶( LDH)、肌酸激酶(CK)的溢出.结论:丹参水提物对缺血/再灌注损伤心脏具有一定保护作用.

Effect of Salvia miltiorrhiza Extract on Heart Rate and Myocardial Enzyme after Myocardial Ischemia/reperfusion Injury in Rats

Objective: To sudy the effect of Salvia miltiorrhiza extract on heart rate and myocardial enzyme after myocardial ischemia/reperfusion injury in rats. Method: The SD rats were randomly divided into six groups(n=8, each): sham-operation group, model group, S. miltiorrhiza extract 12.8,6.3,3.2 mg·kg^-1 groups and S. miltiorrhiza injection 4×10³ mg·kg^-1 groups. The myocardial ischemia/reperfusion injury animal model was established by ligaturing left ventricular branch of coronary artery for 30 min and reperfusion for 30 min. The S. miltiorrhiza extract at 12.8,6.4, 3.2 mg·kg^-1and S. miltiorrhiza injection at crude drug 4.0 g·kg^-1were injected by femoral vein after 25 min myocardial ischemia and before 5 min reperfusion, and observing the changes of heart rate and myocardial enzyme. Result: S. miltiorrhiza extract significantly inhibited the decrease of heart rate and reduced serum aspartate transaminase(AST), lactate dehydrogenase(LDH) and creatine kinase(CK) activity after myocardial ischemia/reperfusion injury in rats. Conclusion: S. miltiorrhiza extract can protect myocardial ischemia/reperfusion injury.