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| 四川藏羌地区五加属主要资源种类的HPLC 指纹图谱特征和种类鉴定 | |
| 引用本文： | 李莹,黄艳菲,刘圆,夏清.四川藏羌地区五加属主要资源种类的HPLC 指纹图谱特征和种类鉴定[J].中国实验方剂学杂志,2012,18(4):142-145. |
| 作者姓名： | 李莹 黄艳菲 刘圆 夏清 |
| 作者单位： | 1. 西南民族大学民族医药研究院,成都610041;四川中医药高等专科学校药品检验系,四川绵阳 621000;成都中医药大学药学院,成都611137 2. 西南民族大学民族医药研究院,成都,610041 3. 四川中医药高等专科学校药品检验系,四川绵阳 ,621000 |
| 基金项目： | 国家科技部支撑计划项目(2012BAI27B07);四川省科技支撑计划(2011SZ0233);中央高校基本科研业务费专项项目优秀科研团队及重大孵化项目(11NZYTH02);四川省杰出青年学术技术带头人后续计划(2011JQ0051);四川省中医药管理局科技专项(2008-32) |
| 摘 要： | 目的:建立四川藏羌地区五加属主要资源种类的HPLC指纹图谱,为鉴别其种类提供依据.方法:采用梯度洗脱方法,以Kromasil C18色谱柱(4.6 mm×150 mm,5μm),柱温30℃,以甲醇-0.5％磷酸水溶液为流动相.建立四川藏羌地区五加属药材的指纹图谱,并采用“中药色谱指纹图谱相似度评价系统”软件进行相似度的计算.结果:建立的方法重现性较好,并根据检测结果确定了12个共有特征峰.应用DPSv3.0统计软件对获得的HPLC数量化特征进行系统聚类分析(组间距离法,欧氏距离),聚类分析结果为S1与S2,S3红毛五加药材的不同药用部位,成分最为接近；其次是S5(糙叶藤五加茎皮)；再其次分别是S4(蜀五加茎皮)、S8(香加皮药材)、S6(刺五加药材)和S7(五加皮药材).结论:所建立的HPLC指纹图谱对四川藏羌地区五加属主要资源种类的鉴别具有参考价值. |
| 关 键 词： | 红毛五加 蜀五加 糙叶藤五加 HPLC指纹图谱 鉴别 |
Studies on Fingerprint and Identification of Main Species of Acanthopanax in Tibetan and Qiang Ethnic Area of Sichuan |
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| LI Ying,HUANG Yan-fei,LIU Yuan and XIA Qing.Studies on Fingerprint and Identification of Main Species of Acanthopanax in Tibetan and Qiang Ethnic Area of Sichuan[J].China Journal of Experimental Traditional Medical Formulae,2012,18(4):142-145. | |
| Authors: | LI Ying HUANG Yan-fei LIU Yuan and XIA Qing |
| Institution: | Ethnic Medicine Institute of Southwest University for Nationalities, Chengdu 610041, China; Department of Traditional Chinese Medicine and Inspection, Sichuan College of Traditional Chinese Medicine(TCM), Mianyang 621000, China; Traditional Chinese Medicine College of Pharmacy, Chengdu University of TCM, Key Laboratory of Ministry of Education for Western Development and Utilization of Chinese Medicinal Materials, Chengdu 611137, China;Ethnic Medicine Institute of Southwest University for Nationalities, Chengdu 610041, China;Ethnic Medicine Institute of Southwest University for Nationalities, Chengdu 610041, China;Department of Traditional Chinese Medicine and Inspection, Sichuan College of Traditional Chinese Medicine(TCM), Mianyang 621000, China |
| Abstract: | Objective:To establish an effective method for identification of main species of Acanthopanax in Tibetan and Qiang Ethnic area of Sichuan by HPLC.Method:The analysis was carried out at 30 ℃ on a Kromasil C18 column eluted with a mobile phase consisted of methanol-0.5% phosphoric acid,and the data were analyzed with‘Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica’software.Result:The established chromatographic fingerprint method was repeatability,among which 12 fingerprint peaks in common were confirmed.The DPSv3.0 statistics software was used for clustering analysis with the clustering method of Betweengroups linkage and Euclidean distance.The analysis result was that S1,S2 and S3 of the different medical part of A.giraldii Harms were mostly similar,then that of S5,S4,S8,S6 and S7 in turn.Conclusion:This chromatographic fingerprint could be used specifically to distinguish the main species of Acanthopanax Miq.in Tibetan and Qiang Ethnic area of Sichuan. |
| Keywords: | Acanthopanax giraldii A setchuenensis A leucorrhigus var fulvescens HPLC fingerprint identification |
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