| 基于七叶一枝花中高含量皂苷类成分的氮、磷、钾配施方案研究 |
| |
| 引用本文： | 威则日沙,刘哲,兰建龙,赵雪莲,海来约布,张绍山,杨正明,刘圆.基于七叶一枝花中高含量皂苷类成分的氮、磷、钾配施方案研究[J].中草药,2021,52(10):3089-3096. |
| |
| 作者姓名： | 威则日沙 刘哲 兰建龙 赵雪莲 海来约布 张绍山 杨正明 刘圆 |
| |
| 作者单位： | 西南民族大学药学院, 四川 成都 610041;四川省羌彝药用资源保护与利用技术工程实验室, 四川 成都 610225;青藏高原民族药用资源保护与利用国家民委重点实验室, 四川 成都 610225;西南民族大学青藏高原研究院, 四川 成都 610041;四川省羌彝药用资源保护与利用技术工程实验室, 四川 成都 610225;青藏高原民族药用资源保护与利用国家民委重点实验室, 四川 成都 610225;四川省羌彝药用资源保护与利用技术工程实验室, 四川 成都 610225;青藏高原民族药用资源保护与利用国家民委重点实验室, 四川 成都 610225;西南民族大学民族医药研究院, 四川 成都 610041 |
| |
| 基金项目： | 国家重点研发计划项目（2018YFC1708005）；四川省科技计划项重点研发项目（2021YFS0043）；西南民族大学中央高校基本科研业务费专项资助（2021PTJS35） |
| |
| 摘 要： | 目的研究氮、磷、钾肥配施对七叶一枝花Parispolyphyllavar.chinensis中皂苷类成分含量的影响,以优化七叶一枝花最佳氮、磷、钾配施方案,为其合理施肥提供科学依据。方法以3因素5水平二次回归通用旋转组合设计安排田间试验,采用UPLC-ELSD法测定七叶一枝花中7种皂苷含量,通过方差分析建立回归方程(经F检验),利用模型分析氮、磷、钾配施对各皂苷含量的影响,使用统计频数法筛选最优施肥方案。结果贡献率分析表明,氮肥对重楼皂苷II、V的贡献率最大,磷肥对重楼皂苷H、纤细薯蓣皂苷的贡献率最大,钾肥对重楼皂苷I、Ⅵ、Ⅶ的贡献率最大。单因素分析表明,氮、磷、钾对重楼皂苷Ⅶ、Ⅵ含量有极显著影响(P0.01);互作效应分析表明,不同浓度的氮肥、磷肥和钾肥的协同作用显著提高重楼皂苷Ⅵ的含量;根据回归模型分析最佳施肥范围,取交集得氮、磷、钾肥最优施肥范围分别为516.39～557.19、2771.70～2848.20、461.61～502.65kg/hm2。结论建立了重楼皂苷与氮、磷、钾肥配施的相关性回归方程模型,有利于七叶一枝花中皂苷类成分含量的提高,为合理施肥提供科学性依据。
|
| 关 键 词： | 七叶一枝花 氮 磷 钾 重楼皂苷VII 重楼皂苷VI 重楼皂苷H 重楼皂苷II 纤细薯蓣皂苷 重楼皂苷I 重楼皂苷V 最优施肥量 |
| 收稿时间： | 2020/11/9 0:00:00 |
Research on NPK application scheme of Paris polyphylla var. chinensis on high contents of saponins |
| |
| WEIZE Ri-sh,LIU Zhe,LAN Jian-long,ZHAO Xue-lian,HAILAI Yue-bu,ZHANG Shao-shan,YANG Zheng-ming,LIU Yuan.Research on NPK application scheme of Paris polyphylla var. chinensis on high contents of saponins[J].Chinese Traditional and Herbal Drugs,2021,52(10):3089-3096. |
| |
| Authors: | WEIZE Ri-sh LIU Zhe LAN Jian-long ZHAO Xue-lian HAILAI Yue-bu ZHANG Shao-shan YANG Zheng-ming LIU Yuan |
| |
| Institution: | School of Pharmacy, Southwest Minzu University, Chengdu 610041, China;Sichuan Laboratory of Qiang-Yi Medicinal Resources Protection and Utilization Technology Engineering, Southwest Minzu University, Chengdu 610225, China;Key Laboratory of Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization of National Ethnic Affairs Commission of the People''s Republic of China, Chengdu 610225, China;Institute of Qinghai-tibetan Plateau, Southwest Minzu University, Chengdu 610041, China;Sichuan Laboratory of Qiang-Yi Medicinal Resources Protection and Utilization Technology Engineering, Southwest Minzu University, Chengdu 610225, China;Key Laboratory of Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization of National Ethnic Affairs Commission of the People''s Republic of China, Chengdu 610225, China; Sichuan Laboratory of Qiang-Yi Medicinal Resources Protection and Utilization Technology Engineering, Southwest Minzu University, Chengdu 610225, China;Key Laboratory of Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization of National Ethnic Affairs Commission of the People''s Republic of China, Chengdu 610225, China;Ethnic Medicine Institute, Southwest Minzu University, Chengdu 610041, China |
| |
| Abstract: | Objective To investigate the effect of NPK application on the contents of saponins in Paris polyphylla var. chinensis, so as to optimize the best NPK application for P. polyphylla var. chinensis, which provided some scientific basis for its rational fertilization. Methods A field experiment was carried out with three factors and five levels second order regression current rotation design, and the contents of seven kinds of saponins were determined by UPLC-ELSD. The regression equation was built via variance analysis with F-test, the effect of NPK application on the content of saponins was analyzed by model analysis, and the optimum NPK application was selected by frequency statistic method. Results Results of contribution rate analysis showed that N fertilizer made the highest contribution to polyphyllin II and V, P fertilizer made the highest contribution to polyphyllin H and gracillin, and K fertilizer made the highest contribution to polyphyllin I, VI and VII. Single factor analysis showed that NPK application had a very significant impact on the content of polyphyllin VII and VI (P<0.01). The interaction effect analysis showed that the synergistic effect of NPK application could significantly increase the content of polyphyllin VII. The optimum NPK application areas were 516.39-557.19 kg/hm2, 2771.70-2848.20 kg/hm2 and 461.61-502.65 kg/hm2, respectively. Conclusion The regression equation model was established between the contents of polyphyllins in P. polyphylla var. chinensis and NPK application, which could be beneficial to increase the contents of saponins in P. polyphylla var. chinensis, and provide a scientific basis for rational fertilization. |
| |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中草药》浏览原始摘要信息 |
| 点击此处可从《中草药》下载免费的PDF全文 |
|
