利用转录组数据挖掘东紫苏单萜生物合成相关基因

;目的 获得东紫苏Elsholtzia bodinieri转录组数据信息,了解东紫苏挥发油单萜生物合成途径.方法 采用Illumina Hiseq 2000高通量测序获得东紫苏的转录组数据,通过对数据进行组装、拼接及注释,挖掘其单萜类化合物代谢途径相关基因.结果 东紫苏2个样品材料测序后共获得13.67 Gb数据,91...

High-throughput transcriptome sequencing of Elsholtzia bodinieri and excavation of genes related to monoterpene biosynthesis

Objective To obtain the transcriptome data of Elsholtzia bodinieri and understand the pathway of monoterpene biosynthesis of essential oil from E. bodinieri. Methods In this study, Illumina Hiseq 2000 high-throughput sequencing was used to obtain the transcriptome data of E. bodinieri, and the genes related to the metabolism of monoterpenoids were mined by assembling, splicing and annotating the data. Results A total of 13.67 GB data and 91 169 356 clean reads were obtained after sequencing of the two sample materials of E. bodinieri, 93 327 unigenes were obtained by Trinity assembly with an average length of 1756 bp. The unigenes obtained from the assembly were blasted with NR, GO, KOG, KEGG and other databases. KEGG metabolic pathway analysis showed that there were two monoterpene metabolic pathways, terpenoid backbone biosynthesis (No. ko00900) and monoterpenoid biosynthesis (No. ko00902), with 11 and 30 related unigenes, respectively. Full-length unigenes of six monoterpene synthesis related candidate genes were successfully verified by RT-PCR and sequencing. Conclusion For the first time, high-throughput transcriptome sequencing analysis of E. bodinieri was carried out, and the key enzyme genes of monoterpene biosynthesis were obtained, which laid a foundation for the study of subsequent gene functions.