首发精神分裂症病人血清微小RNA-24-2、微小RNA-30b的检测水平及意义

目的 检测首发精神分裂症(FES)病人血清中微小RNA-24-2(mir-24-2)、微小RNA-30b(mir-30b)水平,并探讨血清中mir-24-2、mir-30b表达水平与FES发病的关系.方法 前瞻性选取2016年11月至2018年9月于武汉市精神卫生中心就诊的98例FES病人为研究对象(FES组);同期选...

Detection levels and significance of serum mir-24-2 and mir-30b in patients with first-episode schizophrenia

Objective To detect the levels of microRNA-24-2 (mir-24-2) and microRNA-30b (mir-30b) in the serum of patients with first-episode schizophrenia (FES), and to explore the relationships between the expressions of mir-24-2 and mir-30b in the serum and the pathogenesis of FES.Methods Ninety-eight FES patients, who were treated in Wuhan Mental Health Center from November 2016to September 2018, were prospectively selected as the study objects (FES group); 76 healthy people were selected as the control groupat the same time. The general data of the two groups were analyzed. The expression levels of mir-24-2 and mir-30b in serum of the two groups were detected by real-time fluorescent quantitative PCR (qRT-PCR), and the positive and negative symptom scale (PANSS)scores of FES patients were evaluated. The correlations between the levels of serum mir-24-2 and mir-30b and PANSS were analyzed; and the diagnostic values of mir-24-2 and mir-30b in serum for FES were analyzed.Results The levels of mir-24-2 [(1.81±0.58)], mir30b [(1.46±0.37)], PANSS total score [(78.64±12.31) points], positive symptom score [(12.64±3.73) points], negative symptom score[(20.56±4.32) points], cognitive score [(8.73±1.84) points], excitement score [(8.96±2.13) points] and depression score [(6.75±2.06)points] in FES group were significantly higher than those in the control group [(1.00±0.31), (1.00±0.26), (35.42±5.37) points, (4.51±1.12) points, (6.94±1.21) points, (3.86±0.95) points, (4.19±0.38) points, (4.12±1.08) points] (all P<0.05). The levels of mir-24-2 and mir-30b in FES patients were positively correlated with PANSS total score, positive symptom score, negative symptom score, cognitivescore, excitement score and depression score (P < 0.05). The area under curve (AUC) of serum mir-24-2 and mir-30b in diagnosing FES were 0.863 (95% CI: 0.808-0.919) and 0.856 (95% CI: 0.801-0.911). The specificity were 93.4% and 84.2%, the sensitivity were68.4% and 76.5%, respectively. The AUC of the combination in diagnosing FES was 0.936 (95% CI: 0.899-0.973), the specificity was 88.2%, and the sensitivity was 89.8%.Conclusions The levels of serum mir-24-2 and mir-30b are significantly increased in patients with FES and positively correlated with PANSS score. Serum mir-24-2 and mir-30b may be helpful for the diagnosis of the disease and