槐定碱对永久性大脑中动脉栓塞大鼠脑细胞的作用及机制研究

目的 探究槐定碱对永久性大脑中动脉栓塞(permanent middle cerebral artery occlusion,pMCAO)大鼠脑细胞的作用及机制.方法 SD大鼠随机分为对照组、模型组和槐定碱低、中、高剂量(2.5、5.0、10.0 mg/kg)组,给予槐定碱5d后,建立pMCAO大鼠模型.考察槐定碱对p...

Mechanism of sophoridine on cerebral cell in rats with permanent middle cerebral artery occlusion

Objective To explore the effect and mechanism of sophoridine on brain cells in rats with permanent middle cerebral artery occlusion (pMCAO). Methods SD rats were randomly divided into control group, model group, low-, medium- and high- dose (2.5, 5.0, 10.0 mg/kg) sophoridine groups. After ip sophoridine for 5 d, pMCAO rat model was established. Effect of sophoridine on neurological function score in pMCAO rats were investigated; Effect of sophoridine on cerebral infarction rate in pMCAO rats were investigated; Flow cytometry was used to detect the cell apoptosis rate in ischemic penumbra of rats in each group; Hematoxylin-eosin (HE) staining method was used to observe the pathological changes in brain tissue of rats in each group; Western blotting was used to investigate expressions of B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax), and Caspase-3 in brain tissue of rats. Results Compared with model group, neurological deficit symptoms of rats in sophoridine group were improved, cerebral infarction rate was significantly reduced (P < 0.05, 0.01), cell apoptosis rate in ischemic penumbra was significantly reduced (P < 0.05, 0.01), tissue damage was improved, expressions of Caspase-3 and Bax in brain tissue were significantly reduced (P < 0.05, 0.01), and Bcl-2 expression was significantly increased (P < 0.05, 0.01). Conclusion Sophoridine has a protective effect on brain cells in pMCAO rats, its mechanism may be related to up-regulation of Bcl-2 and down-regulation of Bax and Caspase-3 expressions, thereby inhibiting cell apoptosis.