| 人内皮抑素基因的克隆及其在大肠杆菌中的表达 |
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| 引用本文： | 赵跃然,游力,张建,高春义,田志刚.人内皮抑素基因的克隆及其在大肠杆菌中的表达[J].中国生化药物杂志,2000,21(6):271-274. |
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| 作者姓名： | 赵跃然 游力 张建 高春义 田志刚 |
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| 作者单位： | 250062,济南,山东省医学科学院基础医学研究所,山东省肿瘤生物治疗研究中心 |
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| 摘 要： | 目的 :克隆人内皮抑素 (hES)全长cDNA ,并进行其在大肠杆菌表达的研究。方法 :用RT PCR从胎肝RNA中扩增hEScDNA片段 ,构建重组其克隆和融合蛋白表达载体 ,并进行克隆基因的DNA序列分析。结果 :克隆了hEScDNA ,其序列和国外报道一致 ;构建了重组hES融合蛋白表达菌株 ,表达目的蛋白达菌体总蛋白的5 0 %以上。结论 :通过hES基因克隆和表达的研究 ,获得了hES的基因克隆和高效表达菌株
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| 关 键 词： | 内皮抑素 表达 融合蛋白 |
Cloning and Expression of Human Endostatin Gene in E.coli |
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| Zhao Yueran,You Li,Zhang Jian,Gao Chunyi,Tian Zhigang.Cloning and Expression of Human Endostatin Gene in E.coli[J].Chinese Journal of Biochemical Pharmaceutics,2000,21(6):271-274. |
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| Authors: | Zhao Yueran You Li Zhang Jian Gao Chunyi Tian Zhigang |
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| Abstract: | Purpose:The aim is to clone and express recombinant human endostatin(hES)in E.coli . Methods:The hES gene was cloned with RT PCR from human fetal liver total RNA,and sequenced using ABI DNA sequencer.Expression recombinant was constructed,and the hES fusion protein was purified and cleaved with factor Xa.RhES was separated with amylose resin affinity chromatography.Results:The DNA sequence of cloned hES gene was similar to that reported previously,and fusion protein of recombinant hES expressed was above 50% of total bacterial proteins.Conclusion:The hES gene and its recombinant with high level expression were obtained. |
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| Keywords: | Endostatin Expression Fusion protein? |
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