去甲斑蝥素明胶微球肝节段性栓塞的犬实验研究

目的:研究去甲斑蝥素明胶微球肝节段性栓塞术后肝脏的病理变化过程,及此过程中肝肾功能的改变,为临床推广应用提供依据。方法:以健康杂种犬为实验对象(n=8),去甲斑蝥素(NCTD)明胶微球(直径约200μm)与60％泛影葡胺混和液作为栓塞剂,行肝节段性栓塞,术前术后监测血常规、淀粉酶、肝肾功能,术后定期复查肝动脉造影、CT,术后1d及1、2、4周分批处死实验犬进行肝肺组织学检查。结果:术后定期复查肝动脉造影示靶动脉均有不同程度的闭塞,术后病理检查发现肝栓塞区小动脉血栓形成,1周后血栓机化,栓塞肝组织以灶性坏死为主,坏死肝细胞完全崩解,代之以纤维组织增生。术后1d白细胞出现-过性升高,术后2周恢复至术前水平。肝功能呈-过性损害,术后2-3周恢复,主要表现为ALT和ALP的升高。结论:直径为200μm的NCTD明胶微球为一种有效的新型动脉栓塞剂。

Hepatic segmental embolization with norcantharides spongostan micrspheres in dogs

Objective: To study the histological change of liver after embolized segmentally with Norcantharides spongostan microspheres. Methods: Eight dogs were embolized segmentally with norcantharides (NCTD) spongostan microspheres (200μm in diameter). Blood routine, the function of liver and kidney were monitored before and after embolization. CT and hepatic arteri -ography were performed after the procedure. The dogs were killed for histologica] study. Results: The targeted hepatic arteries were found to have been occluded in different grades by hepatic arteriography and to have had thrombosis by pathological examination after embolization. The embolized regions became necrotized afer 4 weeks. The function of liver was abnormal temporarily and recovered to normal 2 to 3 weeks after embolization.Conclusion: NCTD spongostan microspheres of 200μm in diameter is a new embolic material. More research is needed to prove its antitumor effects after being injected into hepatic artery.