|
|||||
|
|
| bFGF和EGF对体外培养新生大鼠海马神经元促生长作用的研究 | |
| 引用本文： | 苏万东,吴承远,辛华,仇红,王建刚.bFGF和EGF对体外培养新生大鼠海马神经元促生长作用的研究[J].中华神经外科杂志,2000,16(2):104-106. |
| 作者姓名： | 苏万东 吴承远 辛华 仇红 王建刚 |
| 作者单位： | 1. 250012,济南,山东医科大学附属医院神经外科 2. 山东医科大学细胞生物学教研室 3. 山东医科大学生理学教研室 |
| 摘 要： | 研究不同浓碱性成纤维细胞生长因子和表皮生长因子对新生大鼠海马神经元生长活性的影响。方法取新生大鼠海马组织进行原代体外分离培养，从细胞形态学方面及应用ＭＴＴ法观察ｂＦＧＦ和ＥＧＦ对大鼠海马神经元的影响。结果ｂＦＧＦ能明显促进体外培养大鼠海马神经元存活及突起生长，ＥＧＦ也能促进其存活，尤以促进突起生长更为明显； |
| 关 键 词： | bFGF 表皮生长因子 海马 神经元 体外培养 |
| 修稿时间： | 1998年8月4日 |
The study of the outgrowth stimulating effects of bFGF and EGF on neonatal rat hippocampal neurons |
|
| SU Wandong,WU Chengyuan,XIN Hua,et al..The study of the outgrowth stimulating effects of bFGF and EGF on neonatal rat hippocampal neurons[J].Chinese Journal of Neurosurgery,2000,16(2):104-106. | |
| Authors: | SU Wandong WU Chengyuan XIN Hua |
| Institution: | SU Wandong,WU Chengyuan,XIN Hua,et al. Department of Neurosurgery,Affiliated Hospital of Shandong Medical University,Jinan 250012 |
| Abstract: | Objective To study the growth effects of different concentrations of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) on neonatal rat hippocampal neurons. Methods Dissociated neurons were primarily cultured in vitro and the effects of bFGF and EGF on hippocampal neurons were observed in cytomorphology and by MTT method.Results bFGF and EGF can significantly enhance the survival and neurite out growth of rat hippocampal neurons. bFGF and EGF can both slow down the aging of the neurons. Both can increase the intracellular dehydrogenase activity of the neurons. Conclusions bFGF and EGF both can enhance the outgrowth of the neonatal rat hippocampal neurons in vitro. |
| Keywords: | bFGF EGF Hippocampus Neuron Cultured in vitro |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |