| 新生儿隐球菌白化突变化分子机制的初步研究 |
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| 引用本文： | 桑红,廖万清,陈江汉,温海. 新生儿隐球菌白化突变化分子机制的初步研究[J]. 医学研究生学报, 2001, 14(2): 103-105 |
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| 作者姓名： | 桑红 廖万清 陈江汉 温海 |
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| 作者单位： | 1. 南京军区南京总医院皮肤科
2. 第二军医大学附属长征医院皮肤科真菌病研究室 |
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| 基金项目： | 国家自然科学基金!资助课题 (编号 :3 9870 65 8) |
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| 摘 要： | 目的：研究新生儿隐球菌白化突变分子机制，方法：采用聚合酶链反应（PCR）技术对新生隐球菌产黑素株与白化突变株进行比较研究，并进行PCR产物的克隆，构建重组表达质粒，转化大肠杆菌。结果：发现白化突变株酚氧化酶结构基因（CNLAC1）在1715－2617bp之间大片段缺失（约450bp),而产黑色素株却无此缺失片菌病的预后和基因治疗提供可能的靶基因。
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| 关 键 词： | 新生儿隐球菌白化突变 分子机制 黑色素 漆酶基因 |
| 文章编号： | 1008-8199(2001)02-0103-03 |
| 修稿时间： | 2000-06-24 |
A preliminary study of the molecular mechanism of Cryptococcus neoformans mutation in the production of melanin |
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| Sang Hong,LIAO Wan-qing,CHEN Jiang-han,WEN Hai. A preliminary study of the molecular mechanism of Cryptococcus neoformans mutation in the production of melanin[J]. Bulletin of Medical Postgraduate, 2001, 14(2): 103-105 |
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| Authors: | Sang Hong LIAO Wan-qing CHEN Jiang-han WEN Hai |
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| Affiliation: | SANG Hong 1,LIAO Wan qing 2,CHEN Jiang han 2,WEN Hai 2 |
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| Abstract: | Objectives：To study the molecular mechanism of Cryptococcus neoformans mutation in the production of melanin.Methods：By comparing wild types(Mel+) and mutants(Mel-),we detected C.neoformans mutant laccase (CNLAC1) gene by PCR.We also cloned gene of the CNLAC1 containing about 450bp in length.E.coli DH5α was the host strain for the plasmid containing the DNA clone.Results：No deletion was found in wild type.However,CNLAC1 deletion (about 450 bp) was present (between 1 715 bp and 2 617 bp).CNLAC1 containing about 450 bp in length was cloned successfully.Conclusions：The present study was helpful in the biochemical and molecular study of CNLAC1 mutation.It might also provide us a target gene for the prognosis and gene therapy of Cryptocosis. |
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| Keywords: | Cryptococcus neoformans Melanin CNLAC1 |
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